Evaluation of nonideality from gel chromatographic partition coefficients. A technique with greater versatility than equilibrium dialysis

Van Damme M.-P.I., Murphy W.H., Comper W.D., Preston B.N. and Winzor D.J. (1989) Evaluation of nonideality from gel chromatographic partition coefficients. A technique with greater versatility than equilibrium dialysis. Biophysical Chemistry, 33 2: 115-125. doi:10.1016/0301-4622(89)80014-6


Author Van Damme M.-P.I.
Murphy W.H.
Comper W.D.
Preston B.N.
Winzor D.J.
Title Evaluation of nonideality from gel chromatographic partition coefficients. A technique with greater versatility than equilibrium dialysis
Journal name Biophysical Chemistry   Check publisher's open access policy
ISSN 0301-4622
Publication date 1989-01-01
Sub-type Article (original research)
DOI 10.1016/0301-4622(89)80014-6
Open Access Status Not yet assessed
Volume 33
Issue 2
Start page 115
End page 125
Total pages 11
Language eng
Subject 1304 Biophysics
1303 Biochemistry
1605 Organic Chemistry
Abstract Frontal gel chromatography has been used to measure partition coefficients which enable a quantitative evaluation of the thermodynamic nonideality of small solutes generated by the presence of high concentrations of macromolecular solutes. Equivalence of results obtained by the present method and by equilibrium dialysis is demonstrated in a comparison of results for dextran sulfate-NaCl and dextran-sorbitol systems. Interaction coefficients obtained for dextran-sorbitol and protein-polyethylene glycol 4000 systems yield results which are in reasonable agreement with those predicted on the statistical-mechanical basis of excluded volume. Because of its greater versatility in regard to the range of systems that may be studied, the frontal gel chromatographic procedure is likely to be of particular value for the quantitative characterization of thermodynamic nonideality arising from excluded volume effects in concentrated mixtures of macromolecular solutes.
Keyword Excluded volume
Gel chromatography
Interaction coefficient
Partition coefficient
Thermodynamic nonideality
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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