Guinea pig serum l-asparaginase: Purification, and immunological relationship to liver l-asparaginase and serum l-asparaginases in other mammals

Zhang N., Clarke F., Di Trapani G., Keough D. and Beacham I. (1995) Guinea pig serum l-asparaginase: Purification, and immunological relationship to liver l-asparaginase and serum l-asparaginases in other mammals. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, 112 4: 607-612. doi:10.1016/0305-0491(95)00106-9


Author Zhang N.
Clarke F.
Di Trapani G.
Keough D.
Beacham I.
Title Guinea pig serum l-asparaginase: Purification, and immunological relationship to liver l-asparaginase and serum l-asparaginases in other mammals
Journal name Comparative Biochemistry and Physiology -- Part B: Biochemistry and   Check publisher's open access policy
ISSN 0305-0491
Publication date 1995-01-01
Sub-type Article (original research)
DOI 10.1016/0305-0491(95)00106-9
Volume 112
Issue 4
Start page 607
End page 612
Total pages 6
Language eng
Subject 1314 Physiology
1303 Biochemistry
1312 Molecular Biology
Abstract l-asparaginase, an enzyme used in the treatment of acute lymphocytic leukemia, is found in the serum of only a few mammalian groups, including the guinea pig and its close relatives in the superfamily Cavioidea. This report describes the purification and characterization of l-asparaginase from guinea pig serum. Antiserum against the purified enzyme cross-reacted with sera from other Cavioidean species but not with mouse serum. Relatively weak cross-reaction with unpurified l-asparaginase in guinea pig liver indicates a significant degree of evolutionary divergence.
Keyword Cavioidea
Dye-ligand chromatography
Guinea pig
Immunoblotting
Isozymes
l-Asparaginase
Preparative gel electrophoresis
Serum
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 22 Aug 2017, 00:13:21 EST by System User