Serum and salivary IgA antibody responses to Saccharomyces cerevisiae, Candida albicans and Streptococcus mutans in orofacial granulomatosis and Crohn's disease

Savage, N. W., Barnard, K., Shirlaw, P. J., Rahman, D., Mistry, M., Escudier, M. P., Sanderson, J. D. and Challacombe, S. J. (2004) Serum and salivary IgA antibody responses to Saccharomyces cerevisiae, Candida albicans and Streptococcus mutans in orofacial granulomatosis and Crohn's disease. Clinical And Experimental Immunology, 135 3: 483-489. doi:10.1111/j.1365-2249.2004.02394.x


Author Savage, N. W.
Barnard, K.
Shirlaw, P. J.
Rahman, D.
Mistry, M.
Escudier, M. P.
Sanderson, J. D.
Challacombe, S. J.
Title Serum and salivary IgA antibody responses to Saccharomyces cerevisiae, Candida albicans and Streptococcus mutans in orofacial granulomatosis and Crohn's disease
Journal name Clinical And Experimental Immunology   Check publisher's open access policy
ISSN 0009-9104
Publication date 2004-01-01
Sub-type Article (original research)
DOI 10.1111/j.1365-2249.2004.02394.x
Open Access Status DOI
Volume 135
Issue 3
Start page 483
End page 489
Total pages 7
Editor M. J. Walport
Place of publication UK
Publisher Blackwell Science Ltd
Language eng
Subject C1
320899 Dentistry not elsewhere classified
730112 Oro-dental and disorders
Abstract Orofacial granulomatosis (OFG) is a condition of unknown aetiology with histological and, in some cases, clinical association with Crohn's disease (CD). However, the exact relationship between OFG and CD remains uncertain. The aim of this study was to determine whether OFG could be distinguished immunologically from CD by comparing non-specific and specific aspects of humoral immunity in serum, whole saliva and parotid saliva in three groups of patients: (a) OFG only (n = 14), (b) those with both oral and gut CD (OFG + CD) (n = 12) and (c) CD without oral involvement (n = 22) and in healthy controls (n = 29). Non-specific immunoglobulin (IgA, SigA, IgA subclasses and IgG) levels and antibodies to whole cells of Saccharomyces cerevisiae, Candida albicans and Streptococcus mutans were assayed by enzyme-linked immunosorbent assay (ELISA) in serum, whole saliva and parotid saliva. Serum IgA and IgA1 and IgA2 subclasses were raised in all patient groups (P < 0.01). Salivary IgA (and IgG) levels were raised in OFG and OFG + CD (P < 0.01) but not in the CD group. Parotid IgA was also raised in OFG and OFG + CD but not in CD. The findings suggest that serum IgA changes reflect mucosal inflammation anywhere in the GI tract but that salivary IgA changes reflect involvement of the oral cavity. Furthermore, the elevated levels of IgA in parotid saliva suggest involvement of the salivary glands in OFG. Serum IgA antibodies to S. cerevisiae were raised markedly in the two groups with gut disease while serum IgA (or IgG) antibodies to C. albicans were elevated significantly in all three patient groups (P < 0.02). No differences were found with antibodies to S. mutans. Whole saliva IgA antibodies to S. cerevisiae (and C. albicans) were raised in the groups with oral involvement. These findings suggest that raised serum IgA antibodies to S. cerevisiae may reflect gut inflammation while raised SIgA antibodies to S. cerevisiae or raised IgA or IgA2 levels in saliva reflect oral but not gut disease. Analysis of salivary IgA and IgA antibodies to S. cerevisiae as well as serum antibodies in patients presenting with OFG may allow prediction of gut involvement.
Keyword Candida Albicans
Mucosal Immunity
Orofacial Granulomatosis
Secretory Iga
Saccharomyces Cerevisiae
Inflammatory-bowel-disease
Melkersson-rosenthal Syndrome
Avium Subsp Paratuberculosis
Parotid-saliva
Bakers-yeast
Mycobacterial
Glycoprotein
Antigens
Strains
Protein
Immunology
Crohn's Disease
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2005 Higher Education Research Data Collection
School of Dentistry Publications
 
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Created: Wed, 15 Aug 2007, 12:57:54 EST