A morphology independent approach for identifying dividing adult neural stem cells in the mouse hippocampus

Harris, Lachlan, Zalucki, Oressia, Oishi, Sabrina, Burne, Thomas H., Jhaveri, Dhanisha J. and Piper, Michael (2017) A morphology independent approach for identifying dividing adult neural stem cells in the mouse hippocampus. Developmental Dynamics, . doi:10.1002/dvdy.24545

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Author Harris, Lachlan
Zalucki, Oressia
Oishi, Sabrina
Burne, Thomas H.
Jhaveri, Dhanisha J.
Piper, Michael
Title A morphology independent approach for identifying dividing adult neural stem cells in the mouse hippocampus
Journal name Developmental Dynamics   Check publisher's open access policy
ISSN 1097-0177
1058-8388
Publication date 2017-08-02
Sub-type Article (original research)
DOI 10.1002/dvdy.24545
Open Access Status File (Author Post-print)
Total pages 7
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Abstract Type 1 adult hippocampal neural stem cells (AH-NSCs) continue to generate neurons throughout life, albeit at a very low rate. The relative quiescence of this population of cells has led to many studies investigating factors that may increase their division. Current methods of identifying dividing AH-NSCs in vivo require the identification and tracing of radial processes back to nuclei within the subgranular zone. However, caveats to this approach include the time-intensive nature of identifying AH-NSCs with such a process, as well as the fact that this approach ignores the relatively more active population of horizontally oriented AH-NSCs that also reside in the subgranular zone.

Here we describe, and then verify using Hes5::GFP mice, that labeling for the cell cycle marker Ki67 and selection against the intermediate progenitor cell marker TBR2 (Ki67(+ve) ; TBR2(-ve) nuclei) is sufficient to identify dividing horizontally and radially oriented AH-NSCs in the adult mouse hippocampus.

These findings provide a simple and accurate way to quantify dividing AH-NSCs in vivo using a morphology-independent approach that will facilitate studies into neurogenesis within the hippocampal stem cell niche of the adult brain. Developmental Dynamics, 2017. © 2017 Wiley Periodicals, Inc.
Keyword Adult neurogenesis
Cerebral cortex
Hippocampus
Neural stem cell
Neurogenesis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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