Bacterial membrane vesicles transport their DNA cargo into host cells

Bitto, Natalie J., Chapman, Ross, Pidot, Sacha, Costin, Adam, Lo, Camden, Choi, Jasmine, D'Cruze, Tanya, Reynolds, Eric C., Dashper, Stuart G., Turnbull, Lynne, Whitchurch, Cynthia B., Stinear, Timothy P., Stacey, Katryn J. and Ferrero, Richard L. (2017) Bacterial membrane vesicles transport their DNA cargo into host cells. Scientific Reports, 7 1: . doi:10.1038/s41598-017-07288-4

Author Bitto, Natalie J.
Chapman, Ross
Pidot, Sacha
Costin, Adam
Lo, Camden
Choi, Jasmine
D'Cruze, Tanya
Reynolds, Eric C.
Dashper, Stuart G.
Turnbull, Lynne
Whitchurch, Cynthia B.
Stinear, Timothy P.
Stacey, Katryn J.
Ferrero, Richard L.
Title Bacterial membrane vesicles transport their DNA cargo into host cells
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2017-08-01
Sub-type Article (original research)
DOI 10.1038/s41598-017-07288-4
Open Access Status DOI
Volume 7
Issue 1
Total pages 11
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Subject 1000 General
Abstract Bacterial outer membrane vesicles (OMVs) are extracellular sacs containing biologically active products, such as proteins, cell wall components and toxins. OMVs are reported to contain DNA, however, little is known about the nature of this DNA, nor whether it can be transported into host cells. Our work demonstrates that chromosomal DNA is packaged into OMVs shed by bacteria during exponential phase. Most of this DNA was present on the external surfaces of OMVs, with smaller amounts located internally. The DNA within the internal compartments of Pseudomonas aeruginosa OMVs were consistently enriched in specific regions of the bacterial chromosome, encoding proteins involved in virulence, stress response, antibiotic resistance and metabolism. Furthermore, we demonstrated that OMVs carry DNA into eukaryotic cells, and this DNA was detectable by PCR in the nuclear fraction of cells. These findings suggest a role for OMV-associated DNA in bacterial-host cell interactions and have implications for OMV-based vaccines.
Keyword Multidisciplinary
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID APP1030243
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
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