Effects of cycloheximide on B-chronic lymphocytic leukaemic and normal lymphocytes in vitro: Induction of apoptosis

Collins R.J., Harmon B.V., Souvlis T., Pope J.H. and Kerr J.F.R. (1991) Effects of cycloheximide on B-chronic lymphocytic leukaemic and normal lymphocytes in vitro: Induction of apoptosis. British Journal of Cancer, 64 3: 518-522. doi:10.1038/bjc.1991.341


Author Collins R.J.
Harmon B.V.
Souvlis T.
Pope J.H.
Kerr J.F.R.
Title Effects of cycloheximide on B-chronic lymphocytic leukaemic and normal lymphocytes in vitro: Induction of apoptosis
Journal name British Journal of Cancer   Check publisher's open access policy
ISSN 1532-1827
Publication date 1991-01-01
Sub-type Article (original research)
DOI 10.1038/bjc.1991.341
Open Access Status Not yet assessed
Volume 64
Issue 3
Start page 518
End page 522
Total pages 5
Language eng
Subject 2730 Oncology
1306 Cancer Research
Abstract A number of reports indicate that protein synthesis is a requirement for the occurrence of apoptosis. In this study, the effect of the protein synthesis inhibitor cycloheximide (CHM) on spontaneous apoptosis of B-chronic lymphocytic leukaemia (B-CLL) cells, previously shown to occur when they are cultured in RPMI-1640 medium with autologous or heterologous serum, was examined. No definite inhibition of apoptosis was observed. Indeed, CHM-treatment augmented apoptosis in the B-CLL cultures and also induced apoptosis of cultured normal peripheral blood lymphocytes. Augmentation was dose-dependent for B-CLL cells over the concentration range 10m (0.28 fig ml) to 10m (2800 μg ml), resulting in 9% to 98% apoptosis respectively by 24 h of culture (r = 0.619, P = 0.0008). Normal lymphocytes were affected by CHM over the range 10 M to 10 M, resulting in 7% to 74% apoptosis respectively (r = 0.794, P = 0.0001). Inhibition of protein synthesis in these cells by CHM was virtually complete at a concentration of 10 M. The findings are in accord with some recent reports indicating that suppression of protein synthesis by CHM does not inhibit apoptosis in all circumstances. They also illustrate the marked susceptibility of B-CLL cells, compared with normal lymphocytes, to the induction of apoptosis by this drug. The manner in which CHM triggers apoptosis of some cell types is at present uncertain.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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