The equine major plasma serpin multigene family: Partial characterization including sequence of the reactive-site regions

Patterson S.D., Bell K. and Shaw D.C. (1991) The equine major plasma serpin multigene family: Partial characterization including sequence of the reactive-site regions. Biochemical Genetics, 29 9-10: 477-499. doi:10.1007/BF00554047


Author Patterson S.D.
Bell K.
Shaw D.C.
Title The equine major plasma serpin multigene family: Partial characterization including sequence of the reactive-site regions
Journal name Biochemical Genetics   Check publisher's open access policy
ISSN 0006-2928
Publication date 1991-10-01
Sub-type Article (original research)
DOI 10.1007/BF00554047
Open Access Status
Volume 29
Issue 9-10
Start page 477
End page 499
Total pages 23
Publisher Kluwer Academic Publishers-Plenum Publishers
Language eng
Subject 1303 Biochemistry
1105 Ecology, Evolution, Behavior and Systematics
1312 Molecular Biology
1311 Genetics
Abstract The equine Pi system, which is highly polymorphic and was considered to be controlled by a single locus, has been shown to be controlled by four loci (named Spi 1-4). This system is the equine equivalent of the major human plasma serpin (serine protease inhibitor), human αPI. Twenty-two haplotypes of the equine Pi system have been characterized by two-dimensional electrophoresis, resulting in the assignment of p I, M, and bovine trypsin and chymotrypsin inhibition characteristics to 109 proteins. These proteins have been analyzed further to determine their relatedness to each other as well as to human αPI using immunochemical, structural, and functional criteria. The amino acid sequences of the N termini and reactive-site regions have been determined on proteins from each of the four equine Spi loci. This allowed the designation of the proteins from the Spi 1 locus as being METserpins and the functional equivalents of human αPI. The Spi 4 proteins are ARGserpins, and by alignment the Spi 2 proteins are ILEserpins, the first so far described. The P residue for the Spi 3 proteins was unable to be determined. The limited peptide and immunopeptide mapping revealed that proteins from all four loci were closely related, but within the four there were two pairs (Spi 1 and 2 and Spi 3 and 4) which were more related. All were probably derived from the same gene that gave rise to human αPI.
Keyword Equus caballus
protein blotting
pulsed liquid phase sequencing
serpin
two-dimensional electrophoresis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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