Screening and Monitoring Coeliac Disease: Multicentre Trial of a New Serum Antibody Test Kit

Devine P.L., Birrell G.W., Golder J.P., Marsh M.N., Morgan S., Chang G., Gillis D., Hobson P., Robertson P., Whybin R. and Skerritt J.H. (1994) Screening and Monitoring Coeliac Disease: Multicentre Trial of a New Serum Antibody Test Kit. Disease Markers, 12 1: 71-80. doi:10.1155/1994/285298


Author Devine P.L.
Birrell G.W.
Golder J.P.
Marsh M.N.
Morgan S.
Chang G.
Gillis D.
Hobson P.
Robertson P.
Whybin R.
Skerritt J.H.
Title Screening and Monitoring Coeliac Disease: Multicentre Trial of a New Serum Antibody Test Kit
Journal name Disease Markers   Check publisher's open access policy
ISSN 1875-8630
Publication date 1994-01-01
Sub-type Article (original research)
DOI 10.1155/1994/285298
Open Access Status DOI
Volume 12
Issue 1
Start page 71
End page 80
Total pages 10
Language eng
Subject 1312 Molecular Biology
1311 Genetics
1308 Clinical Biochemistry
2704 Biochemistry, medical
Abstract A multicentre trial was conducted to evaluate a new test for anti-gliadin antibodies (AGA) in serum (Coeliac Screening Kit, CSK, Medical Innovations Limited, Artarmon, NSW, Australia). The test showed excellent reproducibility for both anti-gliadin IgA and IgG detection. The average intraassay coefficient of variation (CV) was 3.0% for IgA and 2.4% for IgG (n=6), while the average interassay CV was 6.4% for IgA and 4.3% for IgG (n=3). By defining a positive test as both IgA and IgG elevated, a sensitivity of 93% in untreated coeliacs (n=75) was observed. The corresponding specificities in healthy adults (n=l 30) and healthy children (n=77) were >99% and 100% respectively, while in patients with other gastrointestinal disorders (disease controls) the specificity was 94% (n= 129). The test was also useful in monitoring patients, with anti-gliadin IgA and IgG falling for up to a year after commencing a gluten-free diet (GFD) (12 adults). In some patients however, antibody levels did not reach the normal cutpoint after many months on a GFD, which may reflect the patients’ poor adherence to their gluten free diet. The test was superior to the Pharmacia anti-gliadin ELISA, and should be useful as an aid to the diagnosis of coeliac disease, as well as in the follow-up of treated patients.
Keyword Anti-gliadin antibody
Coeliac disease
Multicentre trial
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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