Drosophila microRNA modulates viral replication by targeting a homologue of mammalian cJun

Monsanto-Hearne, Verna, Asad, Sultan, Asgari, Sassan and Johnson, Karyn N. (2017) Drosophila microRNA modulates viral replication by targeting a homologue of mammalian cJun. Journal of General Virology, 98 7: 1904-1912. doi:10.1099/jgv.0.000831

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Author Monsanto-Hearne, Verna
Asad, Sultan
Asgari, Sassan
Johnson, Karyn N.
Title Drosophila microRNA modulates viral replication by targeting a homologue of mammalian cJun
Formatted title
Drosophila microRNA modulates viral replication by targeting a homologue of mammalian cJun
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
1465-2099
Publication date 2017-07-08
Year available 2017
Sub-type Article (original research)
DOI 10.1099/jgv.0.000831
Open Access Status Not yet assessed
Volume 98
Issue 7
Start page 1904
End page 1912
Total pages 9
Place of publication London, United Kingdom
Publisher The Microbiology Society
Language eng
Formatted abstract
MicroRNAs (miRNAs) are important regulators of biological processes, including host–virus interaction. This study
investigated the involvement of Drosophila melanogaster miR-8-5p in host–virus interaction. Drosophila flies and cells
challenged with Drosophila C virus (DCV) were found to have lower miR-8-5p abundance compared to uninfected samples.
Lowering miR-8-5p abundance by experimental inhibition of the miRNA led to an increase in viral accumulation, suggesting
that the observed decrease in the miR-8-5p abundance during DCV infection enhances viral replication. miR-8-5p putative
targets were identified and included dJun, a transcription factor gene whose mammalian homologue cJun is induced by
various viruses through kinase activation. Increasing miR-8-5p abundance using miR-8-5p mimics resulted in a decrease in
dJun and GFP reporter levels. Furthermore, when the putative target in dJun was mutated, addition of miR-8-5p mimics did
not result in the same antagonistic effect on dJun. These results show negative regulation of dJun by miR-8-5p and suggest
that an miRNA-mediated pathway is involved in dJun regulation during viral infection. To analyse the role of dJun during DCV
infection, dJun was knocked down in cells prior to DCV infection. Knockdown of dJun decreased DCV replication, providing
evidence that dJun up-regulation that is concomitant with miR-8-5p down-regulation during DCV infection supports viral
replication. These results highlight the role of miRNA in regulating the transcription factor gene dJun and uncover a
previously unrecognized mechanism by which dJun is regulated during host–virus interaction.
Keyword Biotechnology & Applied Microbiology
Virology
Biotechnology & Applied Microbiology
Virology
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biological Sciences Publications
 
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Created: Tue, 01 Aug 2017, 11:54:23 EST by Prof Sassan Asgari on behalf of School of Biological Sciences