Cell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines

Zhang, Guangmei, Asad, Sultan, Khromykh, Alexander A. and Asgari, Sassan (2017) Cell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines. Scientific Reports, 7 1: 6935.1-6935.8. doi:10.1038/s41598-017-07279-5


Author Zhang, Guangmei
Asad, Sultan
Khromykh, Alexander A.
Asgari, Sassan
Title Cell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines
Formatted title
Cell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2017-07-31
Year available 2017
Sub-type Article (original research)
DOI 10.1038/s41598-017-07279-5
Open Access Status DOI
Volume 7
Issue 1
Start page 6935.1
End page 6935.8
Total pages 8
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Formatted abstract
The genus Flavivirus contains more than 70 single-stranded, positive-sense arthropod-borne RNA
viruses. Some faviviruses are particularly medically important to humans and other vertebrates
including dengue virus (DENV), West Nile virus, and yellow fever virus. These viruses are transmitted to
vertebrates by mosquitoes and other arthropod species. Mosquitoes are also infected by insect-specifc
faviviruses (ISFs) that do not appear to be infective to vertebrates. Cell fusing agent virus (CFAV) was
the frst described ISF, which was discovered in an Aedes aegypti cell culture. We found that while CFAV
infection could be signifcantly reduced by application of RNAi against the NS5 gene, removal of the
treatment led to quick restoration of CFAV replication. Interestingly, we found that CFAV infection
signifcantly enhanced replication of DENV, and vice versa, DENV infection signifcantly enhanced
replication of CFAV in mosquito cells. We have shown that CFAV infection leads to increase in the
expression of ribonuclease kappa (RNASEK), which is known to promote infection of viruses that rely
on endocytosis and pH-dependent entry. Knockdown of RNASEK by dsRNA resulted in reduced DENV
replication. Thus, increased expression of RNASEK induced by CFAV is likely to contribute to enhanced
DENV replication in CFAV-infected cells.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biological Sciences Publications
 
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Created: Tue, 01 Aug 2017, 11:46:41 EST by Prof Sassan Asgari on behalf of School of Biological Sciences