Understanding the role of microRNAs in the interaction of Aedes aegypti mosquitoes with an insect-specific flavivirus

Lee, Morris, Etebari, Kayvan, Hall-Mendelin, Sonja, van den Hurk, Andrew F., Hobson-Peters, Jody, Vatipally, Sreenu, Schnettler, Esther, Hall, Roy and Asgari, Sassan (2017) Understanding the role of microRNAs in the interaction of Aedes aegypti mosquitoes with an insect-specific flavivirus. Journal of General Virology, 98 7: 1892-1903. doi:10.1099/jgv.0.000832

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Author Lee, Morris
Etebari, Kayvan
Hall-Mendelin, Sonja
van den Hurk, Andrew F.
Hobson-Peters, Jody
Vatipally, Sreenu
Schnettler, Esther
Hall, Roy
Asgari, Sassan
Title Understanding the role of microRNAs in the interaction of Aedes aegypti mosquitoes with an insect-specific flavivirus
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
1465-2099
Publication date 2017-07-12
Year available 2017
Sub-type Article (original research)
DOI 10.1099/jgv.0.000832
Open Access Status Not yet assessed
Volume 98
Issue 7
Start page 1892
End page 1903
Total pages 12
Place of publication London, United Kingdom
Publisher The Microbiology Society
Language eng
Abstract The Flavivirus genus contains some of the most prevalent vector-borne viruses, such as the dengue, Zika and yellow fever viruses that cause devastating diseases in humans. However, the insect-specific Glade of flaviviruses is restricted to mosquito hosts, albeit they have retained the general features of the genus, such as genome structure and replication. The interactions between insect-specific flaviviruses (ISFs) and their mosquito hosts are largely unknown. Pathogenic flaviviruses are known to modulate host-derived microRNAs (miRNAs), a class of non-coding RNAs that are important in controlling gene expression. Alterations in miRNAs may represent changes in host gene expression and promote understanding of virus-host interactions. The role of miRNAs in ISF-mosquito interactions is largely unknown. A recently discovered Australian ISF, Palm Creek virus (PCV), has the ability to suppress medically relevant flaviviruses. Here, we investigated the potential involvement of miRNAs in PCV infection using the model mosquito Aedes aegypti. By combining small-RNA sequencing and bioinformatics analysis, differentially expressed miRNAs were determined. Our results indicated that PCV infection hardly affects host miRNAs. Out of 101 reported miRNAs of Ae. aegypti, only aae-miR-2940-5p had a significantly altered expression over the course of infection. However, further analysis of aae-miR-2940-5p revealed that this miRNA does not have any direct impact on PCV replication in vitro. Thus, overall the results suggest that PCV infection has a limited effect on the mosquito miRNA profile and therefore miRNAs may not play a significant role in the PCV-Ae. aegypti interaction.
Formatted abstract
The Flavivirus genus contains some of the most prevalent vector-borne viruses, such as the dengue, Zika and yellow fever
viruses that cause devastating diseases in humans. However, the insect-specific clade of flaviviruses is restricted to
mosquito hosts, albeit they have retained the general features of the genus, such as genome structure and replication. The
interactions between insect-specific flaviviruses (ISFs) and their mosquito hosts are largely unknown. Pathogenic flaviviruses
are known to modulate host-derived microRNAs (miRNAs), a class of non-coding RNAs that are important in controlling gene
expression. Alterations in miRNAs may represent changes in host gene expression and promote understanding of virus–host
interactions. The role of miRNAs in ISF–mosquito interactions is largely unknown. A recently discovered Australian ISF, Palm
Creek virus (PCV), has the ability to suppress medically relevant flaviviruses. Here, we investigated the potential involvement
of miRNAs in PCV infection using the model mosquito Aedes aegypti. By combining small-RNA sequencing and bioinformatics
analysis, differentially expressed miRNAs were determined. Our results indicated that PCV infection hardly affects host
miRNAs. Out of 101 reported miRNAs of Ae. aegypti, only aae-miR-2940-5p had a significantly altered expression over the
course of infection. However, further analysis of aae-miR-2940-5p revealed that this miRNA does not have any direct impact
on PCV replication in vitro. Thus, overall the results suggest that PCV infection has a limited effect on the mosquito miRNA
profile and therefore miRNAs may not play a significant role in the PCV–Ae. aegypti interaction.
Keyword Palm Creek virus
microRNA
mosquito
insect-specific flavivirus
piRNA
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID APP1062983
MC_UU_120141/1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biological Sciences Publications
 
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Created: Tue, 01 Aug 2017, 11:40:14 EST by Prof Sassan Asgari on behalf of School of Biological Sciences