Automated high-throughput in vitro screening of the acetylcholine esterase inhibiting potential of environmental samples, mixtures and single compounds

Froment, Jean, Thomas, Kevin V. and Tollefsen, Knut Erik (2016) Automated high-throughput in vitro screening of the acetylcholine esterase inhibiting potential of environmental samples, mixtures and single compounds. Ecotoxicology and Environmental Safety, 130 74-80. doi:10.1016/j.ecoenv.2016.04.005


Author Froment, Jean
Thomas, Kevin V.
Tollefsen, Knut Erik
Title Automated high-throughput in vitro screening of the acetylcholine esterase inhibiting potential of environmental samples, mixtures and single compounds
Journal name Ecotoxicology and Environmental Safety   Check publisher's open access policy
ISSN 1090-2414
0147-6513
Publication date 2016-08-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.ecoenv.2016.04.005
Open Access Status Not yet assessed
Volume 130
Start page 74
End page 80
Total pages 7
Place of publication Maryland Heights, MO United States
Publisher Academic Press
Language eng
Subject 2310 Pollution
2739 Public Health, Environmental and Occupational Health
2307 Health, Toxicology and Mutagenesis
Abstract A high-throughput and automated assay for testing the presence of acetylcholine esterase (AChE) inhibiting compounds was developed, validated and applied to screen different types of environmental samples. Automation involved using the assay in 96-well plates and adapting it for the use with an automated workstation. Validation was performed by comparing the results of the automated assay with that of a previously validated and standardised assay for two known AChE inhibitors (paraoxon and dichlorvos). The results show that the assay provides similar concentration-response curves (CRCs) when run according to the manual and automated protocol. Automation of the assay resulted in a reduction in assay run time as well as in intra- and inter-assay variations. High-quality CRCs were obtained for both of the model AChE inhibitors (dichlorvos IC50=120 µM and paraoxon IC50=0.56 µM) when tested alone. The effect of co-exposure of an equipotent binary mixture of the two chemicals were consistent with predictions of additivity and best described by the concentration addition model for combined toxicity. Extracts of different environmental samples (landfill leachate, wastewater treatment plant effluent, and road tunnel construction run-off) were then screened for AChE inhibiting activity using the automated bioassay, with only landfill leachate shown to contain potential AChE inhibitors. Potential uses and limitations of the assay were discussed based on the present results.
Keyword AChE assay
AChE inhibitors
Automated bioassay
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 290100
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Alliance for Environmental Health Sciences (QAEHS) Publications
 
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