A novel photosensitization treatment for the inactivation of fungal spores and cells mediated by curcumin

Al-Asmari, Fahad, Mereddy, Ram and Sultanbawa, Yasmina (2017) A novel photosensitization treatment for the inactivation of fungal spores and cells mediated by curcumin. Journal of Photochemistry and Photobiology B: Biology, 173 301-306. doi:10.1016/j.jphotobiol.2017.06.009


Author Al-Asmari, Fahad
Mereddy, Ram
Sultanbawa, Yasmina
Title A novel photosensitization treatment for the inactivation of fungal spores and cells mediated by curcumin
Journal name Journal of Photochemistry and Photobiology B: Biology   Check publisher's open access policy
ISSN 1873-2682
1011-1344
Publication date 2017-08-01
Sub-type Article (original research)
DOI 10.1016/j.jphotobiol.2017.06.009
Open Access Status Not yet assessed
Volume 173
Start page 301
End page 306
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
The global concerns regarding the emergence of fungicide-resistant strains and the impact of the excessive use of fungicidal practises on our health, food, and environment have increased, leading to a demand for alternative clean green technologies as treatments. Photosensitization is a treatment that utilises a photosensitiser, light and oxygen to cause cell damage to microorganisms. The effect of photosensitization mediated by curcumin on Aspergillus niger, Aspergillus flavus, Penicillium griseofulvum, Penicillium chrysogenum, Fusarium oxysporum, Candida albicans and Zygosaccharomyces bailii was investigated using three methods. The viability of spores/cells suspended in aqueous buffer using different concentrations of curcumin solution (100–1000 μM) and light dose (0, 24, 48, 72 and 96 J/cm2) were determined. Spraying curcumin solution on inoculated surfaces of agar plates followed by irradiation and soaking spores/cells in curcumin solution prior to irradiation was also investigated. In aqueous mixtures, photosensitised spores/cells of F. oxysporum and C. albicans were inhibited at all light doses and curcumin concentrations, while inactivation of A. niger, A. flavus P. griseofulvum, P. chrysogenum and Z. bailii were highly significant (P < 0.001) reduced by 99%, 88.9%, 78%, 99.7% and 99.2% respectively. On the surface of agar plates, spores/cells exposed to a light dose of 360 J/cm2 sprayed with curcumin at 800 μM showed complete inhibition for A. niger, F. oxysporum, C. albicans and Z. bailii, while A. flavus P. griseofulvum, and P. chrysogenum reduced by 75%, 80.4% and 88.5% respectively. Soaking spores/cells with curcumin solution prior to irradiation did not have a significant effect on the percentage reduction. These observations suggest that a novel photosensitization mediated curcumin treatment is effective against fungal spores/cells and the variation of percentage reduction was dependent on curcumin concentration, light dosage and fungal species.
Keyword Curcumin
Fungi spores/cells
Inactivation
Photosensitization
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Alliance for Agriculture and Food Innovation
 
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Created: Mon, 31 Jul 2017, 09:03:40 EST by Anthony Yeates on behalf of Learning and Research Services (UQ Library)