Enhanced production of para-hydroxybenzoic acid by genetically engineered Saccharomyces cerevisiae

Averesch, Nils J. H., Prima, Alex and Kroemer, Jens O. (2017) Enhanced production of para-hydroxybenzoic acid by genetically engineered Saccharomyces cerevisiae. Bioprocess and Biosystems Engineering, 40 8: 1283-1289. doi:10.1007/s00449-017-1785-z


Author Averesch, Nils J. H.
Prima, Alex
Kroemer, Jens O.
Title Enhanced production of para-hydroxybenzoic acid by genetically engineered Saccharomyces cerevisiae
Journal name Bioprocess and Biosystems Engineering   Check publisher's open access policy
ISSN 1615-7605
1615-7591
Publication date 2017-08-01
Sub-type Article (original research)
DOI 10.1007/s00449-017-1785-z
Open Access Status Not yet assessed
Volume 40
Issue 8
Start page 1283
End page 1289
Total pages 7
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Subject 1305 Biotechnology
1502 Bioengineering
Abstract Saccharomyces cerevisiae is a popular organism for metabolic engineering; however, studies aiming at over-production of bio-replacement precursors for the chemical industry often fail to overcome proof-of-concept stage. When intending to show real industrial attractiveness, the challenge is twofold: formation of the target compound must be increased, while minimizing the formation of side and by-products to maximize titer, rate and yield. To tackle these, the metabolism of the organism, as well as the parameters of the process, need to be optimized. Addressing both we show that S. cerevisiae is well-suited for over-production of aromatic compounds, which are valuable in chemical industry and are particularly useful in space technology. Specifically, a strain engineered to accumulate chorismate was optimized for formation of para-hydroxybenzoic acid. Then a fed-batch bioreactor process was developed, which delivered a final titer of 2.9 g/L, a maximum rate of 18.625 mg/(g × h) and carbon-yields of up to 3.1 mg/g.
Formatted abstract
Saccharomyces cerevisiae is a popular organism for metabolic engineering; however, studies aiming at over-production of bio-replacement precursors for the chemical industry often fail to overcome proof-of-concept stage. When intending to show real industrial attractiveness, the challenge is twofold: formation of the target compound must be increased, while minimizing the formation of side and by-products to maximize titer, rate and yield. To tackle these, the metabolism of the organism, as well as the parameters of the process, need to be optimized. Addressing both we show that S. cerevisiae is well-suited for over-production of aromatic compounds, which are valuable in chemical industry and are particularly useful in space technology. Specifically, a strain engineered to accumulate chorismate was optimized for formation of para-hydroxybenzoic acid. Then a fed-batch bioreactor process was developed, which delivered a final titer of 2.9 g/L, a maximum rate of 18.625 mgpHBA/(gCDW × h) and carbon-yields of up to 3.1 mgpHBA/gglucose.
Keyword Fed-batch
Para-hydroxybenzoic acid
Saccharomyces cerevisiae
Shikimate pathway
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID DE120101549
Institutional Status UQ

 
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