Observations on the blood of the Australian lungfish, neoceratodus forsteri Kief ft II. enzyme cytochemistry of blood cells, peritoneal macrophages and melano-macrophages

Hine P.M., Wain J.M. and Lester R.J.G. (1990) Observations on the blood of the Australian lungfish, neoceratodus forsteri Kief ft II. enzyme cytochemistry of blood cells, peritoneal macrophages and melano-macrophages. Australian Journal of Zoology, 38 2: 105-116. doi:10.1071/ZO9900145


Author Hine P.M.
Wain J.M.
Lester R.J.G.
Title Observations on the blood of the Australian lungfish, neoceratodus forsteri Kief ft II. enzyme cytochemistry of blood cells, peritoneal macrophages and melano-macrophages
Journal name Australian Journal of Zoology   Check publisher's open access policy
ISSN 1446-5698
Publication date 1990-01-01
Sub-type Article (original research)
DOI 10.1071/ZO9900145
Open Access Status Not yet assessed
Volume 38
Issue 2
Start page 105
End page 116
Total pages 12
Language eng
Subject 1105 Ecology, Evolution, Behavior and Systematics
1103 Animal Science and Zoology
Abstract Peroxidase, alkaline and acid phosphatases, esterases, neutral proteases and PAS reactivity were examined in the peripheral blood leucocytes and peritoneal and melano-macrophages of the Australian lungfish. Eosinophils contained partly cyanide-inhibited peroxidase, and monocytes and blasts showed weak peroxidase staining, but other blood leucocytes were negative. Only neutrophils and heterophils showed alkaline phosphatase reactivity, but acid phosphatase was ubiquitous in leucocytes and macrophages. Alpha-naphthyl acetate esterase and naphthol AS-D chloroacetate esterase occurred, and were often strong, in all cell types but particularly neutrophils; a-naphthyl butyrate esterase was only observed in monocytes, macrophages, and in the latter stained moderately to strongly. Beta-glucuronidase, β-galactosidase and N-acetyl-β-glucosaminidase were also confined to the monocyte/macrophage lineage. The neutral protease acetyl-L-tyrosine-α-naphthyl esterase was moderate to strong in monocytes and macrophages. All leucocytes except monocytes stained moderately to strongly by pas. Leucocytes showed similarities in enzyme content to those of elasmobranchs, but less so to those of teleosts. They also resembled leucocytes of mammals, particularly in monocyte-macrophage enzyme profiles, in the heterogeneous response for peroxidase, and the restriction of a-naphthyl butyrate esterase to that lineage.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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