Intrinsic circuits in the lateral central amygdala

Hunt, Sarah, Sun, Yajie, Kucukdereli, Hakan, Klein, Ruediger and Sah, Pankaj (2017) Intrinsic circuits in the lateral central amygdala. Eneuro, 4 1: . doi:10.1523/ENEURO.0367-16.2017


Author Hunt, Sarah
Sun, Yajie
Kucukdereli, Hakan
Klein, Ruediger
Sah, Pankaj
Title Intrinsic circuits in the lateral central amygdala
Journal name Eneuro   Check publisher's open access policy
ISSN 2373-2822
Publication date 2017-02-01
Sub-type Article (original research)
DOI 10.1523/ENEURO.0367-16.2017
Open Access Status DOI
Volume 4
Issue 1
Total pages 18
Place of publication Washington, DC, United States
Publisher Society for Neuroscience
Language eng
Abstract Network activity in the lateral central amygdala (CeL) plays a crucial role in fear learning and emotional processing. However, the local circuits of the CeL are not fully understood and have only recently begun to be explored in detail. Here, we characterized the intrinsic circuits in the CeL using paired whole-call patch-clamp recordings, immunohistochemistry, and optogenetics in C57BL/6J wild-type and somatostatin-cre (SOM-Cre) mice. Our results revealed that throughout the rostrocaudal extent of the CeL, neurons form inhibitory connections at a rate of ∼29% with an average amplitude of 20 ± 3 pA (at −40 mV). Inhibitory input from a single neuron is sufficient to halt firing in the postsynaptic neuron. Post hoc immunostaining for protein kinase Cδ (PKCδ) in wild-type mice and paired recordings in SOM-Cre mice demonstrated that the most common local connections were PKCδ(−) → PKCδ(−) and SOM(+) → SOM(+). Finally, by optogenetically activating either SOM(+) or SOM(−) neurons, we found that almost all neurons in the CeL were innervated by these neuronal populations and that connections between like neurons were stronger than those between different neuronal types. These findings reveal a complex network of connections within the CeL and provide the foundations for future behavior-specific circuit analysis of this complex network.
Keyword Anxiety
Emotion
Fear
Learning
Central nucleus
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Brain Institute Publications
 
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