Integrated microfluidic array plate (iMAP) for cellular and molecular analysis

Dimov, Ivan K., Kijanka, Gregor, Park, Younggeun, Ducrée, Jens, Kang, Taewook and Lee, Luke P. (2011) Integrated microfluidic array plate (iMAP) for cellular and molecular analysis. Lab on a Chip, 11 6: 2701-2710. doi:10.1039/c1lc20105k

Author Dimov, Ivan K.
Kijanka, Gregor
Park, Younggeun
Ducrée, Jens
Kang, Taewook
Lee, Luke P.
Title Integrated microfluidic array plate (iMAP) for cellular and molecular analysis
Journal name Lab on a Chip   Check publisher's open access policy
ISSN 1473-0197
Publication date 2011-08-21
Sub-type Article (original research)
DOI 10.1039/c1lc20105k
Open Access Status Not yet assessed
Volume 11
Issue 6
Start page 2701
End page 2710
Total pages 10
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Language eng
Formatted abstract
Just as the Petri dish has been invaluable to the evolution of biomedical science in the last 100 years, microfluidic cell assay platforms have the potential to change significantly the way modern biology and clinical science are performed. However, an evolutionary process of creating an efficient microfluidic array for many different bioassays is necessary. Specifically for a complete view of a cell response it is essential to incorporate cytotoxic, protein and gene analysis on a single system. Here we present a novel cellular and molecular analysis platform, which allows access to gene expression, protein immunoassay, and cytotoxicity information in parallel. It is realized by an integrated microfluidic array plate (iMAP). The iMAP enables sample processing of cells, perfusion based cell culture, effective perturbation of biologic molecules or drugs, and simultaneous, real-time optical analysis for different bioassays. The key features of the iMAP design are the interface of on-board gravity driven flow, the open access input fluid exchange and the highly efficient sedimentation based cell capture mechanism (∼100% capture rates). The operation of the device is straightforward (tube and pump free) and capable of handling dilute samples (5-cells per experiment), low reagent volumes (50 nL per reaction), and performing single cell protein and gene expression measurements. We believe that the unique low cell number and triple analysis capabilities of the iMAP platform can enable novel dynamic studies of scarce cells.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Mater Research Institute-UQ (MRI-UQ)
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Created: Wed, 19 Jul 2017, 13:47:37 EST by Gregor Kijanka on behalf of Mater Research Institute-UQ