Improved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes

Bilgo, Etienne, Lovett, Brian, Fang, Weiguo, Bende, Niraj, King, Glenn F., Diabate, Abdoulaye and St Leger, Raymond J. (2017) Improved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes. Scientific Reports, 7 1: . doi:10.1038/s41598-017-03399-0


Author Bilgo, Etienne
Lovett, Brian
Fang, Weiguo
Bende, Niraj
King, Glenn F.
Diabate, Abdoulaye
St Leger, Raymond J.
Title Improved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2017-06-13
Sub-type Article (original research)
DOI 10.1038/s41598-017-03399-0
Open Access Status DOI
Volume 7
Issue 1
Total pages 8
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Formatted abstract
The continued success of malaria control efforts requires the development, study and implementation of new technologies that circumvent insecticide resistance. We previously demonstrated that fungal pathogens can provide an effective delivery system for mosquitocidal or malariacidal biomolecules. Here we compared genes from arthropod predators encoding insect specific sodium, potassium and calcium channel blockers for their ability to improve the efficacy of Metarhizium against wild-caught, insecticide-resistant anophelines. Toxins expressed under control of a hemolymph-specific promoter increased fungal lethality to mosquitoes at spore dosages as low as one conidium per mosquito. One of the most potent, the EPA approved Hybrid (Ca++/K+ channel blocker), was studied for pre-lethal effects. These included reduced blood feeding behavior, with almost 100% of insects infected with ~6 spores unable to transmit malaria within 5 days post-infection, surpassing the World Health Organization threshold for successful vector control agents. Furthermore, recombinant strains co-expressing Hybrid toxin and AaIT (Na+ channel blocker) produced synergistic effects, requiring 45% fewer spores to kill half of the mosquitoes in 5 days as single toxin strains. Our results identify a repertoire of toxins with different modes of action that improve the utility of entomopathogens as a technology that is compatible with existing insecticide-based control methods.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
 
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