Use of restriction fragment length polymorphisms to study genetic relationships between Australian and Japanese isolates of Phytophthora vignae

E.C.Y.Liew E.C.Y.LiewE.C.Y.Liew E.LiewE. C.Y.https://api.elsevier.com/content/author/author_id/6701444301 (1991) Use of restriction fragment length polymorphisms to study genetic relationships between Australian and Japanese isolates of Phytophthora vignae. Australian Journal of Botany, 39 4: 335-346. doi:10.1071/BT9910335


Author E.C.Y.Liew E.C.Y.LiewE.C.Y.Liew E.LiewE. C.Y.https://api.elsevier.com/content/author/author_id/6701444301
Title Use of restriction fragment length polymorphisms to study genetic relationships between Australian and Japanese isolates of Phytophthora vignae
Journal name Australian Journal of Botany   Check publisher's open access policy
ISSN 1444-9862
Publication date 1991-01-01
Sub-type Article (original research)
DOI 10.1071/BT9910335
Open Access Status Not yet assessed
Volume 39
Issue 4
Start page 335
End page 346
Total pages 12
Language eng
Subject 1105 Ecology, Evolution, Behavior and Systematics
1110 Plant Science
Abstract The genetic relationships among 10 Australian accessions of Phytophthora vignae (Pv) isolated from cowpea, two Japanese accessions of Pv isolated from adzuki bean and one accession of Phytophthora megasperma f. sp. glycinea (Pmg) isolated from soybean were assessed using Restriction Fragment Length Polymorphisms (RFLPs). Using five high-copy probes derived from genomic libraries of Pv and Pmg, RFLP banding patterns used to calculate the genetic distance, d, between isolates, showed that Pmg was very dissimilar to Pv (d = 0.102). In contrast, a close similarity among different isolates of Pv was observed (maximum d = 0.0039), with most polymorphisms being detected using probe pPmgS63. A dendrogram showed that the Japanese and Australian isolates of Pv each belonged to different clusters, with a similar range of intracluster genetic variation between members of the Australian cluster (d range 0.0001-0.0019) compared to the Japanese cluster (d = 0.0022). Because the genetic distance separating the Australian and Japanese clusters (average d = 0.0033) was less than twice that of the intracluster distances, it is proposed that these two geographically isolated groups of P. vignae have recently been derived from a single (unknown) source, rather than each being indigenous pathogens of their respective hosts cowpea and adzuki bean.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 11 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 11 Jul 2017, 00:05:23 EST by Web Cron