An assessment of genetic relationships between members of the phytophthora megasperma complex and phytophthora vignae using molecular markers

S.C.Whisson S.C.WhissonS.C.Whisson S.WhissonS. C.https://api.elsevier.com/content/author/author_id/6603339400 (1993) An assessment of genetic relationships between members of the phytophthora megasperma complex and phytophthora vignae using molecular markers. Australian Systematic Botany, 6 4: 295-308. doi:10.1071/SB9930295


Author S.C.Whisson S.C.WhissonS.C.Whisson S.WhissonS. C.https://api.elsevier.com/content/author/author_id/6603339400
Title An assessment of genetic relationships between members of the phytophthora megasperma complex and phytophthora vignae using molecular markers
Journal name Australian Systematic Botany   Check publisher's open access policy
ISSN 1030-1887
Publication date 1993-01-01
Sub-type Article (original research)
DOI 10.1071/SB9930295
Open Access Status Not yet assessed
Volume 6
Issue 4
Start page 295
End page 308
Total pages 14
Language eng
Subject 1105 Ecology, Evolution, Behavior and Systematics
1110 Plant Science
Abstract Genetic relationships between Phytophthora megasperma f. sp. glycinea (Pmg) and morphologically similar taxa, P. megasperma f. sp. medieaginis (Pmm), P. megasperma f. sp. trifolii (Pmt), P. megasperma from Douglas Fir (PmDF) and asparagus (PmAS) and Phytophthora vignae, were explored by restriction fragment length polymorphism (RFLP) analysis of nuclear DNA using random genomic multi-copy, cDNA, and ribosomal DNA probes as well as random amplified polymorphic DNA (RAPDs) and RFLP analysis of ribosomal intergenic spacer regions amplified by the polymerase chain reaction (PCR). Each method detected large differences between these taxa and P. megasperma f. sp. glycinea. P. vignae was more closely related to P. megasperma f. sp. glycinea than the other taxa on the basis of the cDNA RFLPs and RFLPs of PCR amplified rDNA intergenic spacer regions. We conclude that each of the taxa examined represent separate species. This supports the most recent re- classification based On mitochondrial RFLPs and electrophoretic protein pattems of the host-specific taxa to P. sojae (Pmg), P. trifolii (Pmt) and P. medieaginis (Pmm).
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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