Some aspects of the immune response of koalas (Phascolarctos cinereus) and in vitro neutralization of chlamydia psittaci (koala strains)

A.A.Girjes A.A.GirjesAdeeb A.A.A.Girjes A.GirjesAdeeb A.https://api.elsevier.com/content/author/author_id/6603828657 (1993) Some aspects of the immune response of koalas (Phascolarctos cinereus) and in vitro neutralization of chlamydia psittaci (koala strains). Fems Immunology and Medical Microbiology, 6 1: 21-30. doi:10.1016/0928-8244(93)90028-3


Author A.A.Girjes A.A.GirjesAdeeb A.A.A.Girjes A.GirjesAdeeb A.https://api.elsevier.com/content/author/author_id/6603828657
Title Some aspects of the immune response of koalas (Phascolarctos cinereus) and in vitro neutralization of chlamydia psittaci (koala strains)
Journal name Fems Immunology and Medical Microbiology   Check publisher's open access policy
ISSN 1574-695X
Publication date 1993-01-01
Year available 1993
Sub-type Article (original research)
DOI 10.1016/0928-8244(93)90028-3
Open Access Status Not yet assessed
Volume 6
Issue 1
Start page 21
End page 30
Total pages 10
Place of publication AMSTERDAM
Publisher ELSEVIER SCIENCE BV
Language eng
Abstract Western-blot analysis was used to study the reaction of koala antisera, two specific polyclonal antibodies and one monoclonal antibody, with chlamydial antigens in koalas infected with Chlamydia psittaci. The koala sera recognized four C. psittaci surface antigens, corresponding to the major outer membrane protein (39.5 kDa), 31 kDa protein, 18 kDa protein and lipopolysaccharide. The S25-23 LPS specific monoclonal antibody inhibited chlamydial infection (55-67%) with both koala strains (type I and type II). Both koala antiserum and rabbit polyclonal antibodies against either type of chlamydia significantly reduced the number of infected cells resulting from type II infections at a dilution of 1 in 20. Rabbit antiserum against type II was effective in neutralizing infection by type II elementary bodies, but was less effective against type I infection. In addition, no koala antiserum was effective in neutralizing type I infection.
Keyword Chlamydia psittaci antigen
Immunoblotting
Koala immune response
Neutralization, in vitro
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 12 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 11 Jul 2017, 00:05:23 EST by Web Cron