A profile of auditory-responsive neurons in the larval zebrafish brain

Vanwalleghem, Gilles, Heap, Lucy A. and Scott, Ethan K. (2017) A profile of auditory-responsive neurons in the larval zebrafish brain. The Journal of Comparative Neurology, 525 14: 3031-3043. doi:10.1002/cne.24258

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Author Vanwalleghem, Gilles
Heap, Lucy A.
Scott, Ethan K.
Title A profile of auditory-responsive neurons in the larval zebrafish brain
Journal name The Journal of Comparative Neurology   Check publisher's open access policy
ISSN 1096-9861
0021-9967
Publication date 2017-06-23
Year available 2017
Sub-type Article (original research)
DOI 10.1002/cne.24258
Open Access Status Not yet assessed
Volume 525
Issue 14
Start page 3031
End page 3043
Total pages 13
Place of publication Hoboken, NJ United States
Publisher John Wiley & Sons
Language eng
Subject 2800 Neuroscience
Abstract Many features of auditory processing are conserved among vertebrates, but the degree to which these pathways are established at early stages is not well explored. In this study, we have observed single cell activity throughout the brains of larval zebrafish with the goal of identifying the cellular responses, brain regions, and brain-wide pathways through which these larvae perceive and process auditory stimuli. Using GCaMP and selective plane illumination microscopy, we find strong responses to auditory tones ranging from 100 Hz to 400 Hz. We also identify different categories of auditory neuron with distinct frequency response profiles. Auditory responses occur in the medial octavolateral nucleus, the torus semicircularis, the medial hindbrain, and the thalamus, and the flow of information among these regions resembles the pathways described in adult fish and mammals. The details of these patterns, however, indicate that auditory processing is still rudimentary in larvae. The range of frequencies detected is small, and while different neurons have distinct response profiles, most are sensitive to multiple frequencies, and distinct categories show substantial overlap in their responses. Likewise, while there are signs of nascent spatial representations of frequency in the larval brain, this only faintly resembles the clear tonotopy seen in adult fish and mammals. Overall, our results show that many fundamental properties of the auditory system are established early in development, and suggest that zebrafish will provide a good model in which to study the development and refinement of these pathways.
Keyword Auditory Pathways
Cochlear Nucleus
Fluorescence
RRID: AB_2315112
RRID: AB_2534077
RRID: SCR_001622
RRID: SCR_001905
RRID: SCR_002234
RRID: SCR_002285
RRID: SCR_002798
RRID: SCR_007198
Zebrafish
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID APP1066887
FT110100887
336331
DP140102036
LE130100078
Institutional Status UQ

 
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