Characterisation of three ACC synthase gene family members during post-harvest-induced senescence in broccoli (Brassica oleracea L. var. italica)

Gonzalez, N. and Botella, J. R. (2003) Characterisation of three ACC synthase gene family members during post-harvest-induced senescence in broccoli (Brassica oleracea L. var. italica). Journal of Plant Biology, 46 4: 223-230. doi:10.1007/BF03030368


Author Gonzalez, N.
Botella, J. R.
Title Characterisation of three ACC synthase gene family members during post-harvest-induced senescence in broccoli (Brassica oleracea L. var. italica)
Journal name Journal of Plant Biology   Check publisher's open access policy
ISSN 1226-9239
1867-0725
Publication date 2003-12-01
Year available 2003
Sub-type Article (original research)
DOI 10.1007/BF03030368
Open Access Status
Volume 46
Issue 4
Start page 223
End page 230
Total pages 8
Place of publication Puchon, South Korea
Publisher Botanical Society of Korea
Language eng
Subject C1
270800 Biotechnology
620000 - Plant Production and Plant Primary Products
Abstract We investigated the gene expression profiles of different members of the 1-aminocyclopropane-1-carboxilic acid (ACC) synthase (EC 4.4.1.14) gene family in broccoli (Brassica oleracea L. var. italica) during the post-harvest-induced senescence process. Using RT-PCR, three different cDNAs coding for ACC synthase (BROCACS1, BROCACS2 and BROCACS3) were amplified from floret tissue at the start of the senescence process. The three genes share relatively little homology, but have highly homologous sequences in Arabidopsis thaliana, and could be functionally related to these counterparts. Southern analyses suggest that BROCACS1 and BROCACS3 are present as single copy genes, while there are probably two copies of BROCACS2. All three genes showed different expression patterns: BROCACS1 is likely to be either wound - or mechanical stress-induced showing high transcript levels after harvesting, but no detectable expression afterwards. BROCACS2 shows steady expression throughout senescence, increasing at the latest stages, and BROCACS3 is almost undetectable until the final stages. Our results suggest that BROCACS1 could be required to initiate the senescence process, while BROCACS2 would be the main ACC synthase gene involved throughout the post-harvest-induced senescence. BROCACS3's expression pattern indicates that it is not directly involved in the initial stages of senescence, but in the final remobilization of cellular resources.
Keyword Plant Sciences
Acc Synthase
Brassica Oleracea
Broccoli
Ethylene
Post-harvest
Senescence
Encoding 1-aminocyclopropane-1-carboxylate Synthase
Arabidopsis-thaliana
Oxidase Gene
Flower Senescence
Leaf Senescence
Acid Synthase
Expression
Cloning
Identification
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 12:23:18 EST