Characterisation of Rph24: a gene conferring adult plant resistance to Puccinia hordei in barley

Ziems, Laura A., Hickey, Lee T., Platz, Gregory J., Franckowiak, Jerome D., Dracatos, Peter M., Singh, Davinder and Park, Robert F. (2017) Characterisation of Rph24: a gene conferring adult plant resistance to Puccinia hordei in barley. Phytopathology, 107 7: 834-841. doi:10.1094/PHYTO-08-16-0295-R


Author Ziems, Laura A.
Hickey, Lee T.
Platz, Gregory J.
Franckowiak, Jerome D.
Dracatos, Peter M.
Singh, Davinder
Park, Robert F.
Title Characterisation of Rph24: a gene conferring adult plant resistance to Puccinia hordei in barley
Formatted title
Characterisation of Rph24: a gene conferring adult plant resistance to Puccinia hordei in barley
Journal name Phytopathology   Check publisher's open access policy
ISSN 0031-949X
1943-7684
Publication date 2017-07-01
Sub-type Article (original research)
DOI 10.1094/PHYTO-08-16-0295-R
Open Access Status Not yet assessed
Volume 107
Issue 7
Start page 834
End page 841
Total pages 8
Place of publication St. Paul, MN, United States
Publisher American Phytopathological Society
Language eng
Formatted abstract
We identified Rph24 as a locus in barley (Hordeum vulgare L.) controlling adult plant resistance (APR) to leaf rust, caused by Puccinia hordei. The locus was previously reported as a quantitative trait locus in barley line ND24260-1 and named qRphND. We crossed ND24260-1 to the leaf-rust-susceptible standard Gus and determined inheritance patterns in the progeny. For the comparative marker frequency analysis (MFA), resistant and susceptible tails of the F2 were genotyped with Diversity Arrays Technology genotyping-by-sequencing (DArT-Seq) markers. The Rph24 locus was positioned at 55.5 centimorgans on chromosome 6H on the DArT-Seq consensus map. Evaluation of F2:3 families confirmed that a single locus from ND24260-1 conferred partial resistance. The haploblock strongly associated with the Rph24 locus was used to estimate the allele frequency in a collection of 282 international barley cultivars. Rph24 was frequently paired with APR locus Rph20 in cultivars displaying high levels of APR to leaf rust. The markers identified in this study for Rph24 should be useful for marker-assisted selection.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Alliance for Agriculture and Food Innovation
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Fri, 07 Jul 2017, 08:03:15 EST by Lee Hickey on behalf of Centre for Plant Science