Genetic analysis of a plasmid encoding haemocin production in Haemophilus paragallinarum

Terry, Tamsin D., Zalucki, Yaramah M., Walsh, Shannon L., Blackall, P. J. and Jennings, Michael P. (2003) Genetic analysis of a plasmid encoding haemocin production in Haemophilus paragallinarum. Microbiology, 149 3177-3184. doi:10.1099/mic.0.26493-0


Author Terry, Tamsin D.
Zalucki, Yaramah M.
Walsh, Shannon L.
Blackall, P. J.
Jennings, Michael P.
Title Genetic analysis of a plasmid encoding haemocin production in Haemophilus paragallinarum
Formatted title
Genetic analysis of a plasmid encoding haemocin production in Haemophilus paragallinarum
Journal name Microbiology   Check publisher's open access policy
ISSN 1350-0872
1465-2080
Publication date 2003-01-01
Year available 2003
Sub-type Article (original research)
DOI 10.1099/mic.0.26493-0
Open Access Status Not Open Access
Volume 149
Start page 3177
End page 3184
Total pages 8
Place of publication Reading
Publisher Society for General Microbiology
Language eng
Subject C1
270301 Bacteriology
780105 Biological sciences
Formatted abstract
The full sequence of plasmid p250, isolated from Haemophilus paragallinarum strain HP250, has been obtained. The plasmid contains seven ORFs: a putative integrase, a putative replication protein (repB) and five ORFs similar to those from the haemocin (bacteriocin) hmcDCBAI operon from Haemophilus influenzae. Of 19 other non-plasmid-containing H. paragallinarum strains screened (11 serovar reference strains and 8 field isolates), 17 strains produced haemocin and were resistant to killing by strain HP250. These strains, unlike strain HP250, have a chromosomally encoded haemocin operon. A number of other members of the family Pasteurellaceae were tested for haemocin sensitivity. Pasteurella avium, Pasteurella volantium and Pasteurella species A, all non-pathogenic bacteria found in the respiratory tract of chickens suffering from respiratory diseases, were sensitive to H. paragallinarum haemocin. However, amongst the pathogenic Pasteurellaceae, 50 % of P. multocida isolates and all five isolates of Pasteurella haemolytica tested were sensitive to the haemocin. Given the prevalence of haemocin production in H. paragallinarum strains, it may play a role in aiding colonization by inhibiting other Gram-negative bacteria that are associated with the respiratory tract in chickens. The origin of replication from plasmid p250 has been used to generate an Escherichia coliH. paragallinarum shuttle vector which may be useful in genetically manipulating H. paragallinarum.
© 2003 SGM
Keyword Microbiology
Gram-negative Bacteria
Hemophilus-paragallinarum
Escherichia-coli
Influenzae
Peptide
Cloning
System
Scheme
Tests
Dna
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 11:53:32 EST