Effect of time of harvest of budded virus on the selection of baculovirus FP mutants in cell culture

Lua, Linda H. L. and Reid, Steven (2003) Effect of time of harvest of budded virus on the selection of baculovirus FP mutants in cell culture. Biotechnology Progress, 19 1: 238-242. doi:10.1021/bp025577u


Author Lua, Linda H. L.
Reid, Steven
Title Effect of time of harvest of budded virus on the selection of baculovirus FP mutants in cell culture
Journal name Biotechnology Progress   Check publisher's open access policy
ISSN 8756-7938
Publication date 2003-01-01
Year available 2003
Sub-type Article (original research)
DOI 10.1021/bp025577u
Open Access Status Not yet assessed
Volume 19
Issue 1
Start page 238
End page 242
Total pages 5
Place of publication Washington, DC 20036 USA
Publisher Amer Chemical Soc
Language eng
Subject C1
670602 Crop and animal protection chemicals
270303 Virology
290101 Fermentation, Biotechnology and Industrial Microbiology
Abstract Rapid formation and selection of FP (few polyhedra) mutants occurs during serial passaging of Helicoverpa armigera nucleopolyhedrovirus (HaSNPV) in insect cell culture. The production of HaSNPV for use as biopesticides requires the passaging of the virus over a number of passages to produce enough virus inoculum for large-scale fermentation. During serial passaging in cell culture, FP mutants were rapidly selected, resulting in declined productivity and reduced potency of virus. Budded virus (BV) is usually harvested between 72 and 96 h postinfection (hpi) in order to obtain a high titer virus stock. In this study, the effect of tine of harvest (TOH) for BV on the selection rate of HaSNPV FP mutants during serial passaging was investigated. BV were harvested at different times postinfection, and each series was serially passaged for six passages. The productivity and percentage of FP mutants at each passage were determined. It was found that the selection of FP mutants can he reduced by employing an earlier TOH for BV. Serial passaging with BV harvested at 48 hpi showed a slower accumulation of FP mutants compared to that of BV harvested after 48 hpi. Higher cell specific yields were also maintained when BV were harvested at 48 hpi. When BV that were formed between 48 and 96 hpi were harvested and serially passaged, FP mutants quickly dominated the virus population. This suggests that the V formed and released between 48 and 96 hpi are most likely from FP mutant infected cells.
Keyword Biotechnology & Applied Microbiology
Food Science & Technology
Nuclear Polyhedrosis-virus
Trichoplusia-ni
Serial Passage
Replication
Infectivity
Heliothis
Growth
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 11:52:58 EST