Sensitivity of Helicoverpa armigera nucleopolyhedrovirus polyhedra to sodium dodecyl sulfate

Lua, L. H. L., Nielsen, L. K. and Reid, S. (2003) Sensitivity of Helicoverpa armigera nucleopolyhedrovirus polyhedra to sodium dodecyl sulfate. Biological Control, 26 1: 57-67. doi:10.1016/S1049-9644(02)00116-0


Author Lua, L. H. L.
Nielsen, L. K.
Reid, S.
Title Sensitivity of Helicoverpa armigera nucleopolyhedrovirus polyhedra to sodium dodecyl sulfate
Journal name Biological Control   Check publisher's open access policy
ISSN 1049-9644
Publication date 2003-01-01
Sub-type Article (original research)
DOI 10.1016/S1049-9644(02)00116-0
Volume 26
Issue 1
Start page 57
End page 67
Total pages 11
Place of publication San Diego, Ca 92101-4495 USA
Publisher Academic Press Inc Elsevier Science
Language eng
Subject C1
290101 Fermentation, Biotechnology and Industrial Microbiology
670602 Crop and animal protection chemicals
Abstract Sodium dodecyl sulfate (SDS) is commonly used to extract polyhedra from infected cells and diseased dead larval tissues. It was found, however, that 80% of Helicoverpa armigera nucleopolyhedrovirus (HaSNPV) polyhedra produced via cell culture were damaged after 30 min of 0.5% SDS treatment whereas only 20% of in vivo produced polyhedra were damaged by the same treatment. Transmission and scanning electron microscopy revealed that the damaged polyhedra had lost their polyhedron envelopes and virions were dislodged from the polyhedrin matrix, leaving empty spaces that were previously occupied by the occluded virions. Up to 20% in vitro produced polyhedra were resistant to SDS and remained intact, even after a 24 h exposure to SDS. This sensitivity to SDS was observed across a range of cell culture media, including serum supplemented media. Electron microscopy also revealed that the inferior polyhedron envelope of in vitro produced polyhedra is likely due to poor interaction between the polyhedron envelope, polyhedron envelope protein (PEP), and polyhedrin matrix. The PEP gene was cloned and sequenced and mutations in this gene were ruled out as an explanation. In vitro produced polyhedra that were passed through insect larva once were resistant to SDS, indicating that a critical component is lacking in insect cell culture medium used for producing HaSNPV or the cells growing in culture are inefficient in some ways in relation to production of polyhedra. (C) 2002 Elsevier Science (USA). All rights reserved.
Keyword Biotechnology & Applied Microbiology
Entomology
Helicoverpa Armigera Nucleopolyhedrovirus
Baculovirus
Biopesticide
Polyhedron Envelope
Polyhedron Envelope Protein
Envelope Protein
Virus
Sequence
Genome
Heliothis
Body
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 11:52:56 EST