Dehydration converts DsbG crystal diffraction from low to high resolution

Heras, B., Edeling, M. A., Byriel, K. A., Jones, A., Raina, S. and Martin, J. L. (2003) Dehydration converts DsbG crystal diffraction from low to high resolution. Structure, 11 2: 139-145. doi:10.1016/S0969-2126(03)00005-4


Author Heras, B.
Edeling, M. A.
Byriel, K. A.
Jones, A.
Raina, S.
Martin, J. L.
Title Dehydration converts DsbG crystal diffraction from low to high resolution
Journal name Structure   Check publisher's open access policy
ISSN 0969-2126
Publication date 2003-01-01
Sub-type Article (original research)
DOI 10.1016/S0969-2126(03)00005-4
Volume 11
Issue 2
Start page 139
End page 145
Total pages 7
Editor Hendrickson
Wayne A
Place of publication Cambridge, USA
Publisher Cell Press
Language eng
Subject C1
279999 Biological Sciences not elsewhere classified
780105 Biological sciences
060112 Structural Biology (incl. Macromolecular Modelling)
Abstract Diffraction quality crystals are essential for crystallographic studies of protein structure, and the production of poorly diffracting crystals is often regarded as a dead end in the process. Here we show a dramatic improvement of poorly diffracting DsbG crystals allowing high-resolution diffraction data measurement. Before dehydration, the crystals are fragile and the diffraction pattern is streaky, extending to 10 Angstrom resolution. After dehydration, there is a spectacular improvement, with the diffraction pattern extending to 2 Angstrom resolution. This and other recent results show that dehydration is a simple, rapid, and inexpensive approach to convert poor quality crystals into diffraction quality crystals.
Keyword Biochemistry & Molecular Biology
Biophysics
Cell Biology
Crystal Dehydration
X-ray Diffraction
Dsb Proteins
Disulfide Isomerase
Disulfide Bond Formation
Escherichia-coli
Tetragonal Lysozyme
Chaperone Activity
Formation Invivo
In-vivo
Quality
Crystallization
Isomerase
Pathway
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2004 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Wed, 15 Aug 2007, 11:52:46 EST