A Sco homologue plays a role in defence against oxidative stress in pathogenic Neisseria

Seib, K. L., Jennings, M. P. and McEwan, A. G. (2003) A Sco homologue plays a role in defence against oxidative stress in pathogenic Neisseria. Febs Letters, 546 2-3: 411-415. doi:10.1016/S0014-5793(03)00632-X

Author Seib, K. L.
Jennings, M. P.
McEwan, A. G.
Title A Sco homologue plays a role in defence against oxidative stress in pathogenic Neisseria
Journal name Febs Letters   Check publisher's open access policy
ISSN 0014-5793
Publication date 2003-01-01
Sub-type Article (original research)
DOI 10.1016/S0014-5793(03)00632-X
Open Access Status Not yet assessed
Volume 546
Issue 2-3
Start page 411
End page 415
Total pages 5
Place of publication The Netherlands
Publisher Elsevier Science
Language eng
Subject C1
270301 Bacteriology
780105 Biological sciences
Abstract Sco proteins are found in mitochondria and in a variety of oxidase positive bacteria. Although Sco is required for the formation of the Cu-A centre in a cytochrome oxidase of the aa(3) type, it was observed that oxidases with a Cu-A centre are not present in many bacteria that contain a Sco homologue. Two bacteria of this type are the pathogens Neisseria meningitidis and Neisseria gonorrhoeae. The sco genes of N. gonorrhoeae strain 1291 and N. meningitidis strain MC58 were cloned, inactivated by inserting a kanamycin resistance cassette and used to make knockout mutants by allelic exchange. Both N. gonorrhoeae and N. meningitidis sco mutants were highly sensitive to oxidative killing by paraquat, indicating that Sco is involved in protection against oxidative stress in these bacteria. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
Keyword Biochemistry & Molecular Biology
Cell Biology
Oxidative Stress
Cytochrome Oxidase
Neisseria Gonorrhoeae
Neisseria Meningitidis
Mitochondrial Copper-metabolism
Complete Genome Sequence
Aerobic Respiration
Yeast Sco1
Q-Index Code C1
Additional Notes The Sco protein of mitochondria is considered to provide copper for the assembly of CuA centre of cytochrome oxidase. This paper showed that Sco proteins are present in bacteria that lack a cytochrome oxidase with a CuA centre and demonstrated that the Sco protein was required for defense against oxidative stress. This challenges an orthodoxy for which there is no direct experimental evidence.

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Created: Wed, 15 Aug 2007, 11:48:01 EST