Synthesis of optically complex core-shell colloidal suspensions: Pathways to multiplexed biological screening

Lawrie, GA, Battersby, BJ and Trau, M (2003) Synthesis of optically complex core-shell colloidal suspensions: Pathways to multiplexed biological screening. Advanced Functional Materials, 13 11: 887-896. doi:10.1002/adfm.200304390


Author Lawrie, GA
Battersby, BJ
Trau, M
Title Synthesis of optically complex core-shell colloidal suspensions: Pathways to multiplexed biological screening
Journal name Advanced Functional Materials   Check publisher's open access policy
ISSN 1616-301X
Publication date 2003-01-01
Sub-type Article (original research)
DOI 10.1002/adfm.200304390
Volume 13
Issue 11
Start page 887
End page 896
Total pages 10
Place of publication Weinheim, Germany
Publisher Wiley-Verlag
Language eng
Subject C1
250103 Colloid and Surface Chemistry
780103 Chemical sciences
Abstract The ability to generate enormous random libraries of DNA probes via split-and-mix synthesis on solid supports is an important biotechnological application of colloids that has not been fully utilized to date. To discriminate between colloid-based DNA probes each colloidal particle must be 'encoded' so it is distinguishable from all other particles. To this end, we have used novel particle synthesis strategies to produce large numbers of optically encoded particle suitable for DNA library synthesis. Multifluorescent particles with unique and reproducible optical signatures (i.e., fluorescence and light-scattering attributes) suitable for high-throughput flow cytometry have been produced. In the spectroscopic study presented here, we investigated the optical characteristics of multi-fluorescent particles that were synthesized by coating silica 'core' particles with up to six different fluorescent dye shells alternated with non-fluorescent silica 'spacer' shells. It was observed that the diameter of the particles increased by up to 20% as a result of the addition of twelve concentric shells and that there was a significant reduction in fluorescence emission intensities from inner shells as an increasing number of shells were deposited.
Keyword Chemistry, Physical
Materials Science, Multidisciplinary
Physics, Applied
Physics, Condensed Matter
Monodisperse Silica Spheres
Fluorescent
Fluorophores
Dispersions
Libraries
Size
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 11:47:16 EST