Model-based analysis of anaerobic acetate uptake by a mixed culture of polyphosphate-accumulating and glycogen-accumulating organisms

Zeng, RJ, Yuan, ZG and Keller, J (2003) Model-based analysis of anaerobic acetate uptake by a mixed culture of polyphosphate-accumulating and glycogen-accumulating organisms. Biotechnology And Bioengineering, 83 3: 293-302. doi:10.1002/bit.10671


Author Zeng, RJ
Yuan, ZG
Keller, J
Title Model-based analysis of anaerobic acetate uptake by a mixed culture of polyphosphate-accumulating and glycogen-accumulating organisms
Journal name Biotechnology And Bioengineering   Check publisher's open access policy
ISSN 0006-3592
Publication date 2003-01-01
Sub-type Article (original research)
DOI 10.1002/bit.10671
Volume 83
Issue 3
Start page 293
End page 302
Total pages 10
Editor D.S. Clark
Place of publication United States
Publisher John Wiley & Sons, Inc
Language eng
Subject C1
270399 Microbiology not elsewhere classified
770499 Other
Abstract An increasing number of studies shows that the glycogen-accumulating organisms (GAOs) can survive and may indeed proliferate under the alternating anaerobic/aerobic conditions found in EBPR systems, thus forming a strong competitor of the polyphosphate-accumulating organisms (PAOs). Understanding their behaviors in a mixed PAO and GAO culture under various operational conditions is essential for developing operating strategies that disadvantage the growth of this group of unwanted organisms. A model-based data analysis method is developed in this paper for the study of the anaerobic PAO and GAO activities in a mixed PAO and GAO culture. The method primarily makes use of the hydrogen ion production rate and the carbon dioxide transfer rate resulting from the acetate uptake processes by PAOs and GAOs, measured with a recently developed titration and off-gas analysis (TOGA) sensor. The method is demonstrated using the data from a laboratory-scale sequencing batch reactor (SBR) operated under alternating anaerobic and aerobic conditions. The data analysis using the proposed method strongly indicates a coexistence of PAOs and GAOs in the system, which was independently confirmed by fluorescent in situ hybridization (FISH) measurement. The model-based analysis also allowed the identification of the respective acetate uptake rates by PAOs and GAOs, along with a number of kinetic and stoichiometric parameters involved in the PAO and GAO models. The excellent fit between the model predictions and the experimental data not involved in parameter identification shows that the parameter values found are reliable and accurate. It also demonstrates that the current anaerobic PAO and GAO models are able to accurately characterize the PAO/GAO mixed culture obtained in this study. This is of major importance as no pure culture of either PAOs or GAOs has been reported to date, and hence the current PAO and GAO models were developed for the interpretation of experimental results of mixed cultures. The proposed method is readily applicable for detailed investigations of the competition between PAOs and GAOs in enriched cultures. However, the fermentation of organic substrates carried out by ordinary heterotrophs needs to be accounted for when the method is applied to the study of PAO and GAO competition in full-scale sludges. (C) 2003 Wiley Periodicals, Inc.
Keyword Biotechnology & Applied Microbiology
Polyphosphate-accumulating Organisms (paos)
Glycogen-accumulating Organisms (gaos)
Toga
Metabolic Model
Anaerobic Acetate Uptake
Model Identification
Biological Phosphorus Removal
Activated-sludge Systems
Water Treatment Processes
Stoichiometry
Ph
Identification
Microorganisms
Microbiology
Kinetics
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 11:37:13 EST