Electroporation of binary Ti plasmid vector into Agrobacterium tumefaciens and Agrobacterium rhizogenes

Nagel R., Elliott A., Masel A., Birch R.G. and Manners J.M. (1990) Electroporation of binary Ti plasmid vector into Agrobacterium tumefaciens and Agrobacterium rhizogenes. FEMS Microbiology Letters, 67 3: 325-328. doi:10.1016/0378-1097(90)90017-K


Author Nagel R.
Elliott A.
Masel A.
Birch R.G.
Manners J.M.
Title Electroporation of binary Ti plasmid vector into Agrobacterium tumefaciens and Agrobacterium rhizogenes
Journal name FEMS Microbiology Letters   Check publisher's open access policy
ISSN 1574-6968
Publication date 1990-01-01
Sub-type Article (original research)
DOI 10.1016/0378-1097(90)90017-K
Open Access Status Not yet assessed
Volume 67
Issue 3
Start page 325
End page 328
Total pages 4
Language eng
Subject 2404 Microbiology
1312 Molecular Biology
1311 Genetics
Abstract Efficient transformation of strains of Agrobacterium tumefaciens and Agrobacterium rhizogenes by electroporation with binary Ti plasmid vector is reported. This procedure yields rates of transformation of 10-10 per μg DNA, which is several orders of magnitude greater than previously published procedures for this genus, the efficiency of transformation varies with the bacterial strain used. This procedure will be useful for the construction of plant DNA libraries directly in Agrobacterium.
Keyword Agrobacterium
Electroporation
Plant gene isolation
Ti plasmid vectors
Transformation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 95 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 27 Jun 2017, 00:03:55 EST by System User