Genomic screen for genes involved in mammalian craniofacial development

Fowles, L. F., Bennetts, J. S., Berkman, J. L., Williams, E., Koopman, P., Teasdale, R. D. and Wicking, C. (2003) Genomic screen for genes involved in mammalian craniofacial development. Genesis, 35 2: 73-87. doi:10.1002/gene.10165

Author Fowles, L. F.
Bennetts, J. S.
Berkman, J. L.
Williams, E.
Koopman, P.
Teasdale, R. D.
Wicking, C.
Title Genomic screen for genes involved in mammalian craniofacial development
Journal name Genesis   Check publisher's open access policy
ISSN 1526-954X
Publication date 2003-01-01
Year available 2003
Sub-type Article (original research)
DOI 10.1002/gene.10165
Open Access Status
Volume 35
Issue 2
Start page 73
End page 87
Total pages 15
Place of publication New York, USA
Publisher Wiley-Liss
Language eng
Subject C1
270205 Genetic Development (incl. Sex Determination)
780105 Biological sciences
Abstract Using a subtractive hybridisation approach, we enriched for genes likely to play a role in embryonic development of the mammalian face and other structures. This was achieved by subtracting cDNA derived from adult mouse liver from that derived from 10.5 dpc mouse embryonic branchial arches 1 and 2. Random sequencing of clones from the resultant library revealed that a high percentage correspond to genes with a previously established role in embryonic development and disease, while 15% represent novel or uncharacterised genes. Whole mount in situ hybridisation analysis of novel genes revealed that approximately 50% have restricted expression during embryonic development. In addition to expression in branchial arches, these genes showed a range of expression domains commonly including neural tube and somites. Notably, all genes analysed were found to be expressed not only in the branchial arches but also in the developing limb buds, providing support for the hypothesis that development of the limbs and face is likely to involve analogous molecular processes. (C) 2003 Wiley-Liss, Inc.
Keyword Developmental Biology
Genetics & Heredity
Craniofacial Development
Mouse Embryogenesis
In Situ Hybridisation
Expression Screen
Branchial Arches
Mccune-albright Syndrome
Stimulatory G-protein
Mutational Analysis
Signaling Pathway
Glypican Gene
Q-Index Code C1
Institutional Status UQ

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Created: Wed, 15 Aug 2007, 05:30:49 EST