High irradiance increases organogenesis in friable callus of Caustis blakei Kuk. (Cyperaceae)

Webber, J, Johnston, ME and Wearing, AH (2003) High irradiance increases organogenesis in friable callus of Caustis blakei Kuk. (Cyperaceae). In Vitro Cellular & Developmental Biology-plant, 39 March-April: 139-141. doi:10.1079/IVP2002380

Author Webber, J
Johnston, ME
Wearing, AH
Title High irradiance increases organogenesis in friable callus of Caustis blakei Kuk. (Cyperaceae)
Journal name In Vitro Cellular & Developmental Biology-plant   Check publisher's open access policy
ISSN 1054-5476
Publication date 2003-01-01
Sub-type Article (original research)
DOI 10.1079/IVP2002380
Open Access Status
Volume 39
Issue March-April
Start page 139
End page 141
Total pages 3
Editor Gregory Philips
Eng-Chong Pua
Place of publication Unites States
Publisher Society for In Vitro Biology
Language eng
Subject C1
300301 Plant Improvement (Selection, Breeding and Genetic Engineering)
620209 Ornamentals, Australian natives and nursery plants
Abstract Caustis blakei is an attractive cut foliage plant harvested from the wild in Australia and marketed under the name of koala fern. Previous attempts to propagate large numbers of this plant have been unsuccessful. The effect of four light irradiances on organogenesis from compact and friable callus of C. blakei was studied for 21 wk. Both callus types produced numerous primordial shoots but many failed to develop into green plantlets. However, significantly more primordial shoots and green plantlets developed on the friable callus than on the compact callus, and significantly more green plantlets were regenerated under the higher photon irradiances of 200 and 300 mumol m(-2) s(-1) than under the lower irradiances of 100 and 150 mumol m(-2) s(-1). The compact callus produced its maximum number of green plantlets early in the experiment (after 9 wk), while the friable callus continued to produce primordial shoots and green plantlets throughout the period of the experiment, and reached its maximum production of green plantlets at 21 wk under the irradiance of 300 mumol m(-2) s(-1). Organogenesis from friable callus under high irradiance (300 mumol m(-2) s(-1)) offers an efficient propagation method for C. blakei.
Keyword Plant Sciences
Cell Biology
Developmental Biology
Compact Callus
Friable Callus
Koala Fern
Photon Irradiance
Triticum-aestivum L
Somatic Embryos
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 05:20:25 EST