WDR62 regulates early neural and glial progenitor specification of human pluripotent stem cells

Alshawaf, Abdulah J., Antonic, Ana, Skafidas, Efstratios, Ng, Dominic Chi-Hung and Dottori, Mirella (2017) WDR62 regulates early neural and glial progenitor specification of human pluripotent stem cells. Stem Cells International, 2017 7848932. doi:10.1155/2017/7848932


Author Alshawaf, Abdulah J.
Antonic, Ana
Skafidas, Efstratios
Ng, Dominic Chi-Hung
Dottori, Mirella
Title WDR62 regulates early neural and glial progenitor specification of human pluripotent stem cells
Journal name Stem Cells International   Check publisher's open access policy
ISSN 1687-966X
1687-9678
Publication date 2017-01-01
Sub-type Article (original research)
DOI 10.1155/2017/7848932
Open Access Status DOI
Volume 2017
Start page 7848932
Total pages 9
Place of publication New York, NY, United States
Publisher Hindawi
Language eng
Subject 1312 Molecular Biology
1307 Cell Biology
Abstract Mutations in WD40-repeat protein 62 (WDR62) are commonly associated with primary microcephaly and other developmental cortical malformations. We used human pluripotent stem cells (hPSC) to examine WDR62 function during human neural differentiation and model early stages of human corticogenesis. Neurospheres lacking WDR62 expression showed decreased expression of intermediate progenitor marker, TBR2, and also glial marker, S100β. In contrast, inhibition of c-Jun N-terminal kinase (JNK) signalling during hPSC neural differentiation induced upregulation of WDR62 with a corresponding increase in neural and glial progenitor markers, PAX6 and EAAT1, respectively. These findings may signify a role of WDR62 in specifying intermediate neural and glial progenitors during human pluripotent stem cell differentiation.
Formatted abstract
Mutations in WD40-repeat protein 62 (WDR62) are commonly associated with primary microcephaly and other developmental cortical malformations. We used human pluripotent stem cells (hPSC) to examine WDR62 function during human neural differentiation and model early stages of human corticogenesis. Neurospheres lacking WDR62 expression showed decreased expression of intermediate progenitor marker, TBR2, and also glial marker, S100β. In contrast, inhibition of c-Jun N-terminal kinase (JNK) signalling during hPSC neural differentiation induced upregulation of WDR62 with a corresponding increase in neural and glial progenitor markers, PAX6 and EAAT1, respectively. These findings may signify a role of WDR62 in specifying intermediate neural and glial progenitors during human pluripotent stem cell differentiation.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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