Genes induced by growth hormone in a model of adipogenic differentiation

Shang, C. A., Thompson, B. J. L., Teasdale, R., Brown, R. J. and Waters, M. J. (2002) Genes induced by growth hormone in a model of adipogenic differentiation. Molecular and Cellular Endocrinology, 189 1-2: 213-219. doi:10.1016/S0303-7207(01)00590-1


Author Shang, C. A.
Thompson, B. J. L.
Teasdale, R.
Brown, R. J.
Waters, M. J.
Title Genes induced by growth hormone in a model of adipogenic differentiation
Journal name Molecular and Cellular Endocrinology   Check publisher's open access policy
ISSN 0303-7207
Publication date 2002-01-01
Sub-type Article (original research)
DOI 10.1016/S0303-7207(01)00590-1
Volume 189
Issue 1-2
Start page 213
End page 219
Total pages 7
Editor I. T. Huhtaniemi
W. E. Rainey
Place of publication Ireland
Publisher Elsevier Science Ireland Ltd
Language eng
Subject C1
270201 Gene Expression
780105 Biological sciences
Abstract A substantial number of GH regulated genes have been reported in mature hepatocytes. but genes involved in GH-initiated cell differentiation have not yet been identified. Here we have studied a, ell-characterised model of GH-dependent differentiation, adipogenesis of 3T3-F442A preadipocytes, to identify genes rapidly induced by GH. Using the suppression subtractive hybridisation technique, we have identified eight genes induced within 60 min of GH treatment, and verified these by northern analysis. Six were identifiable as Stat 2. Stat 3, thrombospondin-1. oncostatin M receptor beta chain. a DEAD box RNA helicase. and muscleblind. a developmental transcription factor. Bioinformatic approaches assigned one of the two remaining unknown genes as a novel 436 residue serine,threonine kinase. As each of the identified genes hake important developmental roles. they may be important in initiating GH-induced adipogenesis. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
Keyword Cell Biology
Endocrinology & Metabolism
Growth Hormone
Adipocyte Differentiation
Subtractive Hybridisation
Gene Induction
Ternary Complex Factors
Mouse Oncostatin-m
3t3-f442a Preadipocytes
C-fos
Binding Protein
Dependent Differentiation
Transcription Factor
C/ebp-delta
Factor-i
Cells
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Created: Wed, 15 Aug 2007, 05:02:59 EST