Exogenous Slit2 does not affect ureteric branching or nephron formation during kidney development

Piper, Michael, Nurcombe, Victor, Wilkinson, Lorine and Little, Melissa (2002) Exogenous Slit2 does not affect ureteric branching or nephron formation during kidney development. International Journal of Developmental Biology, 46 4: 545-550.


Author Piper, Michael
Nurcombe, Victor
Wilkinson, Lorine
Little, Melissa
Title Exogenous Slit2 does not affect ureteric branching or nephron formation during kidney development
Journal name International Journal of Developmental Biology   Check publisher's open access policy
ISSN 0214-6282
Publication date 2002-01-01
Year available 2002
Sub-type Article (original research)
Volume 46
Issue 4
Start page 545
End page 550
Total pages 6
Place of publication Spain
Publisher UBC Press
Language eng
Subject C1
270205 Genetic Development (incl. Sex Determination)
730115 Urogenital system and disorders
Abstract In an attempt to elucidate the role of Slit2 invertebrate kidney development, the effect of adding exogenous human Slit2 protein (hSlit2) to developing murine metanephric kidney explants was examined. To confirm the activity of the recombinant Slit2 protein, neurons from 8 day old chick sympathetic nerve chain dorsal root ganglia were cultured with hSlit2 protein, which induced significant neurite branching and outgrowth. Using kidney explants as a model system, metanephric development in the presence of hSlit2 protein was examined. Addition of hSlit2 up to a final concentration of 1 mug/ml had no detectable effect on the formation of nephrons or on branching morphogenesis of the ureteric tree after 2 or 4 days in culture, as assessed via immunofluorescence for the markers WT1 and calbindin 28K respectively. Similarly, maturation of the nephrogenic mesenchyme occurred in a phenotypically normal fashion. In situ analysis of the Slit receptors, Robot and Robot, the vasculogenic markers VEGFA and Flk-1, and the stromal cell marker BF2 displayed no difference in comparison to controls.
Keyword Developmental Biology
Slit2
Metanephros
Mesenchyme
Ureteric Tree
Robo
Repulsive Axon Guidance
Mice Lacking Gdnf
Neuronal Migration
Vertebrate Slit
Lateral Position
Robo Receptors
Nervous-system
Renal Agenesis
Protein
Expression
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 7 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 05:00:22 EST