Enhancement or modulation of the vector competence of ochlerotatus vigilax (Diptera : Culicidae) for Ross River virus by temperature

Kay, B. H. and Jennings, C. D. (2002) Enhancement or modulation of the vector competence of ochlerotatus vigilax (Diptera : Culicidae) for Ross River virus by temperature. Journal of Medical Entomology, 39 1: 99-105. doi:10.1603/0022-2585-39.1.99


Author Kay, B. H.
Jennings, C. D.
Title Enhancement or modulation of the vector competence of ochlerotatus vigilax (Diptera : Culicidae) for Ross River virus by temperature
Formatted title
Enhancement or Modulation of the Vector Competence of Ochlerotatus vigilax (Diptera: Culicidae) for Ross River Virus by Temperature
Journal name Journal of Medical Entomology   Check publisher's open access policy
ISSN 0022-2585
Publication date 2002-01-01
Sub-type Article (original research)
DOI 10.1603/0022-2585-39.1.99
Volume 39
Issue 1
Start page 99
End page 105
Total pages 7
Editor D. D. Steelman
W. K. Reisen
Place of publication United States
Publisher Entomological Society of America
Language eng
Subject C1
321202 Epidemiology
730212 Disease distribution and transmission
Abstract Two different doses of Ross River virus (1111) were fed to Ochlerotatus vigilax (Skuse), the primary coastal vector in Australia; and blood engorged females were held at different temperatures up to 35 d. After ingesting 10(4.3) CCID50/Mosquito, mosquitoes reared at 18 and 25degreesC (and held at the same temperature) had higher body remnant and head and salivary gland titers than those held at 32degreesC, although infection rates were comparable. At 18, 25, and 32degreesC, respectively, virus was first detected in the salivary glands on days 3, 2, and 3. Based on a previously demonstrated 98.7% concordance between salivary gland infection and transmission, the extrinsic incubation periods were estimated as 5, 4, and 3 d, respectively, for these three temperatures. When Oc. vigilax reared at 18, 25, or 32degreesC were fed a lower dosage of 10(3.3) CCID50 RR/mosquito, and assayed after 7 d extrinsic incubation at these (or combinations of these) temperatures, infection rates and titers were similar. However, by 14 d, infection rates and titers of those reared and held at 18 and 32degreesC were significantly higher and lower, respectively. However, this process was reversible when the moderate 25degreesC was involved, and intermediate infection rates and titers resulted. These data indicate that for the strains of RR and Oc. vigilax used, rearing temperature is unimportant to vector competence in the field, and that ambient temperature variations will modulate or enhance detectable infection rates only after 7 d: extrinsic incubation. Because of the short duration of extrinsic incubation, however, this will do little to influence RR epidemiology, because by this time some Oc. vigilax could be seeking their third blood meal, the latter two being infectious.
Keyword Entomology
Veterinary Sciences
Ochlerotatus Vigilax
Ross River Virus
Alphavirus
Vector Competence
Temperature
Australia
Western Equine Encephalomyelitis
Murray Valley Encephalitis
Culex-tarsalis
Aedes-vigilax
Dissemination Barriers
Extrinsic Incubation
California
Mosquitos
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Wed, 15 Aug 2007, 04:58:54 EST