Conserved modularity and potential for alternate splicing in mouse and human Slit genes

Little, Melissa, Rumballe, Bree, Georgas, Kylie, Yamada, Toshiya and Teasdale, Rohan D. (2002) Conserved modularity and potential for alternate splicing in mouse and human Slit genes. International Journal of Developmental Biology, 46 4: 385-391.

Author Little, Melissa
Rumballe, Bree
Georgas, Kylie
Yamada, Toshiya
Teasdale, Rohan D.
Title Conserved modularity and potential for alternate splicing in mouse and human Slit genes
Journal name International Journal of Developmental Biology   Check publisher's open access policy
ISSN 0214-6282
Publication date 2002-01-01
Sub-type Article (original research)
Volume 46
Issue 4
Start page 385
End page 391
Total pages 7
Place of publication Spain
Publisher UBC Press
Language eng
Subject C1
270201 Gene Expression
780105 Biological sciences
Abstract The vertebrate Slit gene family currently consists of three members;Slit1,Slit2 and Slit3. Each gene encodes a protein containing multiple epidermal growth factor and leucine rich repeat motifs, which are likely to have importance in cell-cell interactions. In this study, we sought to fully define and characterise the vertebrate Slit gene family. Using long distance PCR coupled with in silico mapping, we determined the genomic structure of all three Slit genes in mouse and man. Analysis of EST and genomic databases revealed no evidence of further Slit family members in either organism. All three Slit genes were encoded by 36 (Slit3) or 37 (Slit1 and Slit2) exons covering at least 143 kb or 183 kb of mouse or human genomic DNA respectively. Two additional potential leucine-rich repeat encoding exons were identified within intron 12 of Slit2. These could be inserted in frame, suggesting that alternate splicing may occur in Slit2 A search for STS sequences within human Slit3 anchored this gene to D5S2075 at the 5' end (exon 4) and SGC32449 within the 3' UTR, suggesting that Slit3 may cover greater than 693 kb. The genomic structure of all Slit genes demonstrated considerable modularity in the placement of exon-intron boundaries such that individual leucine-rich repeat motifs were encoded by individual 72 by exons. This further implies the potential generation of multiple Slit protein isoforms varying in their number of repeat units. cDNA library screening and EST database searching verified that such alternate splicing does occur.
Keyword Developmental Biology
Leucine-rich Repeat
Epidermal Growth Factor Repeat
In Silico Mapping
Slit Genes
Vertebrate Slit
Extracellular Protein
Expression
Repellent
Motif
Receptors
Egf
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Created: Wed, 15 Aug 2007, 04:57:25 EST