Novel genes regulated by Sonic Hedgehog in pluripotent mesenchymal cells

Ingram, W. J., Wicking, C. A., Grimmond, S. M., Forrest, A. R. and Wainwright, B. J. (2002) Novel genes regulated by Sonic Hedgehog in pluripotent mesenchymal cells. Oncogene, 21 53: 8196-8205. doi:10.1038/sj.onc.1205975

Author Ingram, W. J.
Wicking, C. A.
Grimmond, S. M.
Forrest, A. R.
Wainwright, B. J.
Title Novel genes regulated by Sonic Hedgehog in pluripotent mesenchymal cells
Journal name Oncogene   Check publisher's open access policy
ISSN 0950-9232
Publication date 2002-01-01
Year available 2002
Sub-type Article (original research)
DOI 10.1038/sj.onc.1205975
Open Access Status Not yet assessed
Volume 21
Issue 53
Start page 8196
End page 8205
Total pages 10
Place of publication England
Publisher Nature Publishing Group
Language eng
Subject C1
270201 Gene Expression
780105 Biological sciences
Abstract Sonic Hedgehog is a secreted morphogen involved in patterning a wide range of structures in the developing embryo. Disruption of the Hedgehog signalling cascade leads to a number of developmental disorders and plays a key role in the formation of a range of human cancers. The identification of genes regulated by Hedgehog is crucial to understanding how disruption of this pathway leads to neoplastic transformation. We have used a Sonic Hedgehog (Shh) responsive mouse cell line, C3H/10T1/2, to provide a model system for hedgehog target gene discovery. Following activation of cell cultures with Shh, RNA was used to interrogate microarrays to investigate downstream transcriptional consequences of hedgehog stimulation. As a result 11 target genes have been identified, seven of which are induced (Thrombomodulin, GILZ, BF-2, Nr4a1, IGF2, PMP22, LASP1) and four of which are repressed (SFRP-1, SFRP-2, Mip1-gamma, Amh) by Shh. These targets have a diverse range of putative functions and include transcriptional regulators and molecules known to be involved in regulating cell growth or apoptosis. The corroboration of genes previously implicated in hedgehog signalling, along with the finding of novel targets, demonstrates both the validity and power of the C3H/10T1/2 system for Shh target gene discovery.
Keyword Biochemistry & Molecular Biology
Cell Biology
Genetics & Heredity
Sonic Hedgehog
Downstream Target Genes
Animal Development
Cdna Microarray
Candidate Gene
Human Homolog
Gli Proteins
Mouse Embryo
Floor Plate
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Faculty of Science Publications
Excellence in Research Australia (ERA) - Collection
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Citation counts: TR Web of Science Citation Count  Cited 97 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 15 Aug 2007, 04:55:13 EST