Revisiting the question of limited genetic variation within Schistosoma japonicum

Le, T. H., Blair, D. and McManus, D. P. (2002) Revisiting the question of limited genetic variation within Schistosoma japonicum. Annals of Tropical Medicine And Parasitology, 96 2: 155-164. doi:10.1179/000349802125000268


Author Le, T. H.
Blair, D.
McManus, D. P.
Title Revisiting the question of limited genetic variation within Schistosoma japonicum
Journal name Annals of Tropical Medicine And Parasitology   Check publisher's open access policy
ISSN 0003-4983
Publication date 2002-01-01
Sub-type Article (original research)
DOI 10.1179/000349802125000268
Volume 96
Issue 2
Start page 155
End page 164
Total pages 10
Editor D. Molyneux
K. Wallbanks
Place of publication United Kingdom
Publisher Maney Publishing
Language eng
Subject C1
321299 Public Health and Health Services not elsewhere classified
730299 Public health not elsewhere classified
Abstract Recent electrophoretic data have indicated that Schistosoma japonicum in mainland China may be a species complex, with the existence of a cryptic species being predicted from the analysis of schistosome populations from Sichuan province. To investigate the Sichuan form of S. japonicum, 4.9 kbp of mitochondrial DNA from each of three samples of the parasite from China (two from Sichuan and one from Hunan) and one from Sorsogon in the Philippines were amplified, sequenced and characterized. The sequence data were compared with those from the related South-east Asian species of S. mekongi (Khong Island, Laos) and S. malayensis (Baling, Malaysia) and that from S. japonicum from Anhui (China). At both the nucleotide and amino-acid levels, the variation among the five S. japonicum samples was limited ( < 1%). This was consistent with the conclusions drawn from previous molecular studies, in which minimal variation among S. japonicum populations was also detected. In contrast, S. mekongi and S. malayensis, species recognized as separate but closely related, differ from each other by about 10%, and each differs by 25%-26% from S. japonicum. Phylogenetic trees provided a graphic representation of these differences, showing all S. japonicum sequences to be very tightly clustered and distant from S. mekongi and S. malayensis, the last two being clearly distinct from each other. The results thus indicate no significant intraspecific genetic variation among S. japonicum samples collected from different geographical areas and do not support the idea of a distinct form in Sichuan.
Keyword Public, Environmental & Occupational Health
Parasitology
Tropical Medicine
Peninsular Malaysia
Philippine
Chinese
Strains
Differentiation
Sequence
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 10 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 04:54:38 EST