Isolation and characterization of the proteoglycans synthesized by adult human pulp fibroblasts in vitro

Bartold, PM, Moule, AJ, Li, H and Rigby, P (1995) Isolation and characterization of the proteoglycans synthesized by adult human pulp fibroblasts in vitro. International Endodontic Journal, 28 3: 163-171. doi:10.1111/j.1365-2591.1995.tb00292.x


Author Bartold, PM
Moule, AJ
Li, H
Rigby, P
Title Isolation and characterization of the proteoglycans synthesized by adult human pulp fibroblasts in vitro
Journal name International Endodontic Journal   Check publisher's open access policy
ISSN 1365-2591
Publication date 1995-01-01
Year available 1995
Sub-type Article (original research)
DOI 10.1111/j.1365-2591.1995.tb00292.x
Open Access Status Not yet assessed
Volume 28
Issue 3
Start page 163
End page 171
Total pages 9
Place of publication OXFORD
Publisher BLACKWELL SCIENCE LTD
Language eng
Abstract The proteoglycans synthesized by fibroblasts derived from healthy human adult dental pulps have been isolated and characterized on the basis of their glycosaminoglycan content, molecular size and charge. The proteoglycans were identified by their labelling with [S-35] sulphate and susceptibility to digestion by papain. The sulphated glycosaminoglycans associated with the proteoglycans were identified following specific enzymatic and chemical degradations as chondroitin sulphate, dermatan sulphate and heparan sulphate. Dermatan sulphate and chondroitin sulphate were identified as the major glycosaminoglycans secreted into the medium, whereas chrondroitin sulphate and heparan sulphate were the principal glycosaminoglycans associated with the cell layers. The proteoglycans could be fractionated on the basis of their charge and size into a number of heterogeneous pools. The principal proteoglycans isolated were small and contained either chondroitin sulphate or dermatan sulphate and most likely correspond to decorin and biglycan. Other molecules with features similar to versican and syndecan were also identified.
Keyword fibroblast
glycosaminoglycan
proteoglycan
pulp
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 11 times in Scopus Article | Citations
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Created: Mon, 19 Jun 2017, 10:59:37 EST by Alexander John Moule