Donor variability in the proliferation of human dental pulp fibroblasts

Moule A.J., Li H. and Bartold P.M. (1995) Donor variability in the proliferation of human dental pulp fibroblasts. Australian Dental Journal, 40 2: 110-114. doi:10.1111/j.1834-7819.1995.tb03125.x


Author Moule A.J.
Li H.
Bartold P.M.
Title Donor variability in the proliferation of human dental pulp fibroblasts
Journal name Australian Dental Journal   Check publisher's open access policy
ISSN 1834-7819
Publication date 1995-01-01
Year available 1995
Sub-type Article (original research)
DOI 10.1111/j.1834-7819.1995.tb03125.x
Open Access Status Not yet assessed
Volume 40
Issue 2
Start page 110
End page 114
Total pages 5
Place of publication NORTH SYDNEY NSW
Publisher AUSTRALIAN DENTAL ASSN INC
Language eng
Abstract Fibroblasts were isolated from human dental pulps of healthy third molars from 49 donors of ages ranging from 17 to 68. Significant variability was noted in the success of obtaining primary cultures from these pulps. Variability between the various cultures was also observed in the reliability of maintaining subcultures of the primary cultures as well as recovery from frozen stocks of established cell lines. Of the original 49 explant cultures studied, only three survived long-term passage and freezing. In addition to difficulties and variability in establishing cell lines, the human pulp fibroblasts also showed great variability in proliferative activity which could not be accounted for by donor age, source, or passage number. These findings highlight significant difficulties in establishing reliable human pulp fibroblast cultures and the need for great care in interpreting any in vitro data.
Keyword Dental pulp
donor variability
fibroblasts
proliferation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 16 times in Scopus Article | Citations
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Created: Mon, 19 Jun 2017, 10:57:06 EST by Alexander John Moule