Fabrication and characterization of hydrogels formed from designer coiled-coil fibril-forming peptides

Dexter, A. F., Fletcher, N. L., Creasey, R. G., Filardo, F., Boehm, M. W. and Jack, K. S. (2017) Fabrication and characterization of hydrogels formed from designer coiled-coil fibril-forming peptides. RSC Advances, 7 44: 27260-27271. doi:10.1039/c7ra02811c


Author Dexter, A. F.
Fletcher, N. L.
Creasey, R. G.
Filardo, F.
Boehm, M. W.
Jack, K. S.
Title Fabrication and characterization of hydrogels formed from designer coiled-coil fibril-forming peptides
Journal name RSC Advances   Check publisher's open access policy
ISSN 2046-2069
Publication date 2017-05-01
Sub-type Article (original research)
DOI 10.1039/c7ra02811c
Open Access Status DOI
Volume 7
Issue 44
Start page 27260
End page 27271
Total pages 12
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Language eng
Abstract Hydrogels are soft solids that represent attractive matrices for tissue engineering, wound healing and drug delivery. We previously reported an α-helical peptide, AFD19, that forms fibrils and hydrogels at pH 6, but precipitates under physiological conditions. We now show that a single targeted change in AFD19 yields peptide AFD36, which gels at physiological pH and in the presence of salt. Furthermore, we present a simple method for homogeneous sol–gel conversion through pH titration with sodium bicarbonate followed by loss of carbon dioxide. Chemical and thermal denaturation studies show AFD36 self-assembles to give stable α-helical structures, forming fibrils of 3.8–3.9 nm diameter at pH 4.0–7.0 as shown by small-angle X-ray scattering and atomic force microscopy. An AFD36 gel at 0.35% (w/v) showed an elastic modulus of 350 Pa. Mouse fibroblasts exhibited low cellular toxicity and spread morphologies when grown on the gel as a preliminary proof of principle towards cell culture studies. These peptide gels offer a molecularly simple, biodegradable alternative to polymer-based systems for biomedical applications.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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