Comparisons between bone and cementum compositions and the possible basis for their layered appearances

Cool, S. M., Forwood, M. R., Campbell, P. and Bennett, M. B. (2002) Comparisons between bone and cementum compositions and the possible basis for their layered appearances. Bone, 30 2: 386-392. doi:10.1016/S8756-3282(01)00686-X

Author Cool, S. M.
Forwood, M. R.
Campbell, P.
Bennett, M. B.
Title Comparisons between bone and cementum compositions and the possible basis for their layered appearances
Journal name Bone   Check publisher's open access policy
ISSN 8756-3282
Publication date 2002-02-01
Year available 2002
Sub-type Article (original research)
DOI 10.1016/S8756-3282(01)00686-X
Open Access Status Not yet assessed
Volume 30
Issue 2
Start page 386
End page 392
Total pages 7
Editor Roland Baron
Ernesto Conolis
Gideon Rodan
Place of publication New York
Publisher Elsevier Science Inc
Language eng
Subject C1
730108 Cancer and related disorders
321015 Oncology and Carcinogenesis
Abstract In humans, age estimation from the adult skeleton represents an attempt to determine chronological age based on growth and maturational events. In teeth, such events can be characterized by appositional growth layers in midroot cementum. The purpose of this study was to determine the underlying cause of the layered microstructure of human midroot cementum. Whether cementum growth layers are caused by changes in relative mineralization, collagen packing and/or orientation, or by variations in organic matrix apposition was investigated by subjecting midroot sections of human canine teeth to analysis using polarized light and scanning electron microscopy (SEM). Polarized light was used to examine transverse midroot sections in both mineralized and demineralized states. Mineralized sections were also reexamined following subsequent decollagenization. Polarized light was additionally used in the examination of mineralized sections taken transversely, longitudinally, and obliquely from the same tooth root. From the birefringence patterns it was concluded that collagen orientation does not change with varying section plane. Instead, the mineral phase was most responsible for the birefringence of the cementum. SEM studies suggested that neither collagen packing nor collagen orientation change across the width of the cementum, confirming and validating the results of the polarized light examination. Also, SEM analysis using electron backscatter and the electron probe suggested no changes in the mean atomic number density, calcium, phosphate, and sulfur levels across the width of the cementum. Therefore, we conclude that crystalline orientation and/or size is responsible for the layered appearance of cementum. (Bone 30:386-392; 2002) (C) 2002 by Elsevier Science Inc. All rights reserved.
Keyword Endocrinology & Metabolism
Backscattered Electron Images
Turkey Leg Tendons
Collagen Fibrils
Dental Cementum
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 20 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 15 Aug 2007, 04:17:29 EST