The assembly factor SDHAF2 is dispensable for flavination of the catalytic subunit of mitochondrial complex II in breast cancer cells

Bezawork-Geleta, Ayenachew, Dong, Lanfeng, Rohlena, Jakub and Neuzil, Jiri (2016) The assembly factor SDHAF2 is dispensable for flavination of the catalytic subunit of mitochondrial complex II in breast cancer cells. Journal of Biological Chemistry, 291 41: 21414-21420. doi:10.1074/jbc.C116.755017

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Author Bezawork-Geleta, Ayenachew
Dong, Lanfeng
Rohlena, Jakub
Neuzil, Jiri
Title The assembly factor SDHAF2 is dispensable for flavination of the catalytic subunit of mitochondrial complex II in breast cancer cells
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 1083-351X
Publication date 2016-10-07
Year available 2016
Sub-type Article (original research)
DOI 10.1074/jbc.C116.755017
Open Access Status File (Publisher version)
Volume 291
Issue 41
Start page 21414
End page 21420
Total pages 7
Place of publication Rockville, MD United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Abstract Mitochondrial complex II or succinate dehydrogenase (SDH) is at the crossroads of oxidative phosphorylation and the tricarboxylic acid cycle. It has been shown that Sdh5 (SDHAF2/SDH5 in mammals) is required for flavination of the subunit Sdh1 (SDHA in human cells) in yeast. Here we demonstrate that in human breast cancer cells, SDHAF2/SDH5 is dispensable for SDHA flavination. In contrast to yeast, CRISPR-Cas9 nickase-mediated SDHAF2 KO breast cancer cells feature flavinated SDHA and retain fully assembled and functional complex II, as well as normal mitochondrial respiration. Our data show that SDHA flavination is independent of SDHAF2 in breast cancer cells, employing an alternative mechanism.
Keyword Cancer biology
Cell biology
Mammal
Mitochondria
Mitochondrial respiratory chain complex
SDH assembly factor
SDHA
Complex II assembly
Flavinylation
Mitochondrial complex II
Succinate dehydrogenase
Flavin adenine dinucleotide
Flavination
Assembly factor
SDHAF2
Cancer cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Brain Institute Publications
 
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Created: Mon, 12 Jun 2017, 09:54:30 EST by Kirstie Asmussen on behalf of Queensland Brain Institute