Kainic acid induces 14-3-3 zeta expression in distinct regions of rat brain

van der Brug, MP, Goodenough, S and Wilce, P (2002) Kainic acid induces 14-3-3 zeta expression in distinct regions of rat brain. Brain Research, 956 1: 110-115. doi:10.1016/S0006-8993(02)03487-X


Author van der Brug, MP
Goodenough, S
Wilce, P
Title Kainic acid induces 14-3-3 zeta expression in distinct regions of rat brain
Journal name Brain Research   Check publisher's open access policy
ISSN 0006-8993
Publication date 2002-01-01
Sub-type Article (original research)
DOI 10.1016/S0006-8993(02)03487-X
Open Access Status
Volume 956
Issue 1
Start page 110
End page 115
Total pages 6
Place of publication Amsterdam
Publisher Elsevier Science
Language eng
Subject C1
320000 Medical and Health Sciences
730100 Clinical (Organs, Diseases and Abnormal Conditions)
Abstract Areas of the limbic system of adult male Wistar rats were screened for kainic-acid-induced gene expression. Polymerase-chain-reactionbased differential display identified a 147-bp cDNA fragment, which represented an mRNA that was upregulated in the entorhinal cortex and hippocampus in the kainic-acid-treated animals. The sequence was 97.8% homologous to rat 14-3-3 zeta isoform mRNA. Detailed Northern analysis revealed increased mRNA levels in the entorhinal cortex I h after kainic acid exposure and continued elevation 24 h post-injection in both the entorhinal cortex and hippocampus. Western blot analyses confirmed that the protein product of this gene was also present in increased amounts over the same time period. Immunohistochemistry and terminal transferase-mediated dUTP nick end labelling (TUNEL) detected expression of 14-3-3 protein exclusively in the entorhinal cortex and hippocampus, and only in TUNEL-positive neuronal cells. Expression of the tumor suppressor protein, p53 was also induced by kainate injection, and was co-localized with 14-3-3 zeta protein in selected cells only in the affected brain regions. The increase gene expression of 14-3-3 represents a transcription-mediated response associated with region selective neuronal damage induced by kainic acid. (C) 2002 Elsevier Science B.V. All rights reserved.
Keyword Neurosciences
Kainate
Kainic Acid
Differential Display
14-3-3
Neuronal Damage
Cell-death
Amphipathic Groove
Gene-expression
Dna-binding
14-3-3-proteins
P53
Hippocampus
Localization
Activation
Pathways
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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Created: Wed, 15 Aug 2007, 03:52:53 EST