Regulatory interactions in Arabidopsis thaliana acetohydroxyacid synthase

Lee, Y. T. and Duggleby, R. G. (2002) Regulatory interactions in Arabidopsis thaliana acetohydroxyacid synthase. Febs Letters, 512 1-3: 180-184. doi:10.1016/S0014-5793(02)02253-6


Author Lee, Y. T.
Duggleby, R. G.
Title Regulatory interactions in Arabidopsis thaliana acetohydroxyacid synthase
Journal name Febs Letters   Check publisher's open access policy
ISSN 0014-5793
Publication date 2002-02-01
Sub-type Article (original research)
DOI 10.1016/S0014-5793(02)02253-6
Volume 512
Issue 1-3
Start page 180
End page 184
Total pages 5
Place of publication The Netherlands
Publisher Elsevier Science
Language eng
Subject C1
270108 Enzymes
780105 Biological sciences
0601 Biochemistry and Cell Biology
Abstract Acetohydroxyacid synthase (AHAS; EC 4.1.3.18) contains catalytic and regulatory subunits, the latter being required for sensitivity to feedback regulation by leucine, valine and isoleucine. The regulatory subunit of Arabidopsis thaliana AHAS possesses a sequence repeat and we have suggested preciously that one repeat binds leucine while the second binds valine or isoleucine, with synergy between the two sites. We have mutated four residues in each repeat, based on a model of the regulatory subunit. The data confirm that there are separate leucine and valine/isoleucine sites, and suggest a complex pathway for regulatory signal transmission to the catalytic subunit. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
Keyword Biochemistry & Molecular Biology
Biophysics
Cell Biology
Acetohydroxyacid Synthase
Branched-chain Amino Acid
Enzyme Inhibition
Enzyme Regulation
Subunit
Acetolactate Synthase
Small-subunit
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Wed, 15 Aug 2007, 03:35:08 EST