Callus induction and plant regeneration from broccoli (Brassica oleracea var. italica) for transformation

Kim, J. H and Botella, J. R. (2002) Callus induction and plant regeneration from broccoli (Brassica oleracea var. italica) for transformation. Journal of Plant Biology, 45 3: 177-181. doi:10.1007/BF03030311


Author Kim, J. H
Botella, J. R.
Title Callus induction and plant regeneration from broccoli (Brassica oleracea var. italica) for transformation
Journal name Journal of Plant Biology   Check publisher's open access policy
ISSN 0583-421X
1867-0725
Publication date 2002-09
DOI 10.1007/BF03030311
Volume 45
Issue 3
Start page 177
End page 181
Total pages 5
Place of publication Seoul, Korea
Publisher Botanical Society of Korea
Collection year 2002
Language eng
Subject C1
270800 Biotechnology
770601 Air quality
0607 Plant Biology
Abstract We evaluated the efficiency of callus induction and plantlet regeneration from hypocotyl explants of broccoli (Brassica oleracea var. italica). The cultivars were ‘Marathon’, ‘Greenbelt’, and ‘Shogun’. Transformation success was not affected by the presence of tobacco feeder-cell layers on the culture media. The frequency of shoot regeneration was greater from 10-d-old hypocotyls than from 14-d-old hypocotyls. Both ‘Marathon’ and ‘Greenbelt’ had higher potentials for tissue regeneration than did ‘Shogun’. We found that for transformation selection, the optimum concentration was either 50 mg/L kanamycin or 100 mg/L genetkin.
Keyword broccoli
callus induction
hypocotyls
plant regeneration
transformation
tobacco
feeder
cell
Q-Index Code C1

 
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Created: Tue, 14 Aug 2007, 17:34:45 EST