Short tandem target mimic rice lines uncover functions of miRNAs in regulating important agronomic traits

Zhang, Hui, Zhang, Jinshan, Yan, Jun, Gou, Feng, Mao, Yanfei, Tang, Guiliang, Botella, Jose Ramon and Zhu, Jian-Kang (2017) Short tandem target mimic rice lines uncover functions of miRNAs in regulating important agronomic traits. Proceedings of the National Academy of Sciences, 114 20: 5277-5282. doi:10.1073/pnas.1703752114


Author Zhang, Hui
Zhang, Jinshan
Yan, Jun
Gou, Feng
Mao, Yanfei
Tang, Guiliang
Botella, Jose Ramon
Zhu, Jian-Kang
Title Short tandem target mimic rice lines uncover functions of miRNAs in regulating important agronomic traits
Journal name Proceedings of the National Academy of Sciences   Check publisher's open access policy
ISSN 0027-8424
1091-6490
Publication date 2017-05-16
Year available 2017
Sub-type Article (original research)
DOI 10.1073/pnas.1703752114
Open Access Status Not yet assessed
Volume 114
Issue 20
Start page 5277
End page 5282
Total pages 6
Place of publication Washington, DC, United States
Publisher National Academy of Sciences
Language eng
Subject 1000 General
Abstract Improvements in plant agricultural productivity are urgently needed to reduce the dependency on limited natural resources and produce enough food for a growing world population. Human intervention over thousands of years has improved the yield of important crops; however, it is increasingly difficult to find new targets for genetic improvement. MicroRNAs (miRNAs) are promising targets for crop improvement, but their inactivation is technically challenging and has hampered functional analyses. We have produced a large collection of transgenic short tandem target mimic (STTM) lines silencing 35 miRNA families in rice as a resource for functional studies and crop improvement. Visual assessment of field-grown miRNA-silenced lines uncovered alterations in many valuable agronomic traits, including plant height, tiller number, and grain number, that remained stable for up to five generations. We show that manipulation of miR398 can increase panicle length, grain number, and grain size in rice. In addition, we discovered additional agronomic functions for several known miRNAs, including miR172 and miR156. Our collection of STTM lines thus represents a valuable resource for functional analysis of rice miRNAs, as well as for agronomic improvement that can be readily transferred to other important food crops.
Keyword Small RNA
MicroRNA
Silencing
Yield
Crop
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID R01GM070795
20140029
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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